ReviewMethods in cell biology2010
Photoactivatable green fluorescent protein-tubulin.
Review in Methods in cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed, 4 citations in OpenAlex.
- Mapping Leak Electron Pathways from Mitochondria to the Outer Cell Membrane in Human Embryonic Lung Fibroblasts.Bioelectricity · 2025Article
- Lateral and longitudinal compaction of PRC1 overlap zones drives stabilization of interzonal microtubules.Molecular biology of the cell · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Direct observations of live cells expressing fluorescently tagged tubulin have led to important advances in our understanding of mitosis. A limitation of this approach is that all of the cells' microtubules are fluorescent and thus observation of the behavior of specific subsets of microtubules is precluded. To address this problem, we have tagged tubulin with a photoactivatable variant of green fluorescent protein (PA-GFP), thereby allowing one to follow the behavior of a subset of tagged molecules in the cell. Here, we describe methods to tag and express proteins with PA-GFP, locally photoactivate the recombinant protein and record the dynamic behavior of the photoactivated molecules in live cells. Use of photoactivatable proteins is a powerful approach to examine dynamic processes, including spindle formation, in diverse cells.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.