ArticleNitric oxide : biology and chemistry2010
Direct, real-time measurement of shear stress-induced nitric oxide produced from endothelial cells in vitro.
Article in Nitric oxide : biology and chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 89 citations in OpenAlex.
- Computational Fluid Dynamics Simulation of Endothelium-Modulated Thrombosis.Journal of cardiovascular translational research · 2026Article
- Article
- Mechanical feedback mechanisms in a multiscale sliding filament model of lymphatic muscle pumping.Applications in engineering science · 2025Article
- Buckling of red blood cell membrane in narrow capillaries induces excessive wall shear stress.Biophysical journal · 2025Article
- Nitric Oxide Distribution Correlates with Intraluminal Thrombus in Abdominal Aortic Aneurysm: A Computational Study.Bioengineering (Basel, Switzerland) · 2025Article
- Nitric Oxide Signaling and Sensing in Age-Related Diseases.Antioxidants (Basel, Switzerland) · 2024Review
- Unraveling the complexity of vascular tone regulation: a multiscale computational approach to integrating chemo-mechano-biological pathways with cardiovascular biomechanics.Biomechanics and modeling in mechanobiology · 2024Article
- Abnormal flow pattern of low wall shear stress and high oscillatory shear index in spontaneous vertebral artery dissection with vertebral artery hypoplasia.Frontiers in neuroscience · 2023Article
- Intracellular calcium dynamics of lymphatic endothelial and muscle cells co-cultured in a Lymphangion-Chip under pulsatile flow.The Analyst · 2022Article
- TRPC channel-derived calcium fluxes differentially regulate ATP and flow-induced activation of eNOS.Nitric oxide : biology and chemistry · 2021Article
- Endothelial Dysfunction in Primary Aldosteronism.International journal of molecular sciences · 2019Review
- Hemodynamic Performance of Multilayer Stents in the Treatment of Aneurysms with a Branch Attached.Scientific reports · 2019Article
- Effect of spatial heterogeneity and colocalization of eNOS and capacitative calcium entry channels on shear stress-induced NO production by endothelial cells: A modeling approach.Cellular and molecular bioengineering · 2018Article
- Role of endothelial nitric oxide in control of peripheral vascular conductance during muscle metaboreflex activation.American journal of physiology. Regulatory, integrative and comparative physiology · 2017Article
- Nanomolar nitric oxide concentrations quickly and reversibly modulate astrocytic energy metabolism.The Journal of biological chemistry · 2017Article
- Comparing the Effects of Krebs Plus Verapamil Solution on Endothelial Function of Harvested Human Greater Saphenous Vein with Heparinized Blood, an Invitro Study.Medical archives (Sarajevo, Bosnia and Herzegovina) · 2017Article
- Cholesterol Enrichment Impairs Capacitative Calcium Entry, eNOS Phosphorylation & Shear Stress-Induced NO Production.Cellular and molecular bioengineering · 2017Article
- Patient-Specific, Multi-Scale Modeling of Neointimal Hyperplasia in Vein Grafts.Frontiers in physiology · 2017Article
- Shear-Induced Nitric Oxide Production by Endothelial Cells.Biophysical journal · 2016Article
- Mathematical model for shear stress dependent NO and adenine nucleotide production from endothelial cells.Nitric oxide : biology and chemistry · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Nitric oxide (NO) produced by the endothelium is involved in the regulation of vascular tone. Decreased NO production or availability has been linked to endothelial dysfunction in hypercholesterolemia and hypertension. Shear stress-induced NO release is a well-established phenomenon, yet the cellular mechanisms of this response are not completely understood. Experimental limitations have hindered direct, real-time measurements of NO under flow conditions. We have overcome these challenges with a new design for a parallel-plate flow chamber. The chamber consists of two compartments, separated by a Transwell® membrane, which isolates a NO recording electrode located in the upper compartment from flow effects. Endothelial cells are grown on the bottom of the membrane, which is inserted into the chamber flush with the upper plate. We demonstrate for the first time direct real-time NO measurements from endothelial cells with controlled variations in shear stress. Step changes in shear stress from 0.1 dyn/cm(2) to 6, 10, or 20 dyn/cm(2) elicited a transient decrease in NO followed by an increase to a new steady state. An analysis of NO transport suggests that the initial decrease is due to the increased removal rate by convection as flow increases. Furthermore, the rate at which the NO concentration approaches the new steady state is related to the time-dependent cellular response rather than transport limitations of the measurement configuration. Our design offers a method for studying the kinetics of the signaling mechanisms linking NO production with shear stress as well as pathological conditions involving changes in NO production or availability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.