ReviewCroatian medical journal2010
Review of detection of Brucella spp. by polymerase chain reaction.
Review in Croatian medical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
56 citing papers in PubMed, 1 synthesis or guideline pooled it, 158 citations in OpenAlex.
- What do we really know about brucellosis diagnosis in livestock worldwide? A systematic review.PLoS neglected tropical diseases · 2025Pooled it
- Osteoarticular Infections in Immunocompetent Children Due to Atypical, Fastidious or Unusual Bacterial Pathogens: A Review.Pathogens (Basel, Switzerland) · 2026Review
- Two novel real-time PCR assays forMicrobiology spectrum · 2026Article
- Cervical brucellar spondylitis: Current management strategies and future directions.Infectious medicine · 2026Review
- Development of a rapid and sensitive RPA-CRISPR/Cas12a-based assay for the detection ofMicrobiology spectrum · 2025Article
- Molecular evidence of Brucella abortus circulating in cattle, goats, and humans in Central Equatoria State, South Sudan.Scientific reports · 2025Article
- Molecular and serological survey of Brucella spp. among rodents in western Iran.BMC veterinary research · 2025Article
- Establishment of a 23S rRNA assay for Brucella and its application in evaluating bacterial growth status.Veterinary research communications · 2025Article
- Neurobrucellosis complicated with cerebral venous sinus thrombosis in a young Syrian male: a case report.Annals of medicine and surgery (2012) · 2024Article
- Equine brucellosis in Iran: serological, bacteriological and molecular analysis.Veterinary research communications · 2024Article
- Brucellosis: Unveiling the complexities of a pervasive zoonotic disease and its global impacts.Open veterinary journal · 2024Review
- Polyphasic Characterization ofMicroorganisms · 2024Article
- Brucellosis infection complicated with myelitis: a case report and literature review.Frontiers in cellular and infection microbiology · 2024Review
- Serological Evidence and Coexposure of Selected Infections among Livestock Slaughtered at Eastern Cape Abattoirs in South Africa.International journal of microbiology · 2023Article
- Human brucellosis in Baringo County, Kenya: Evaluating the diagnostic kits used and identifying infecting Brucella species.PloS one · 2023Article
- Evaluation of a Real-time PCR in Combination with a Cultivation Method for the Detection ofJournal of veterinary research · 2022Article
- Isolation and characterisation ofVeterinarni medicina · 2022Article
- Molecular Survey ofArchives of Razi Institute · 2022Article
- Brucella spp. distribution, hosting ruminants from Greece, applying various molecular identification techniques.BMC veterinary research · 2022Article
- Molecular detection of Brucella spp. in ruminant herds in Greece.Tropical animal health and production · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we present a review of most of the currently used polymerase chain reaction (PCR)-based methods for identification of Brucella bacteria in biological samples. We focused in particular on methods using single-pair primers, multiplex primers, real-time PCRs, PCRs for marine Brucella, and PCRs for molecular biotyping. These methods are becoming very important tools for the identification of Brucella, at the species level and recently also at the biovar level. These techniques require minimum biological containment and can provide results in a very short time. In addition, genetic fingerprinting of isolates aid in epidemiological studies of the disease and its control. PCR-based methods are more useful and practical than conventional methods used to identify Brucella spp., and new methods for Brucella spp. identification and typing are still being developed. However, the sensitivity, specificity, and issues of quality control and quality assurance using these methods must be fully validated on clinical samples before PCR can be used in routine laboratory testing for brucellosis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.