ArticleThe Journal of membrane biology2010
Calcium dynamics during physiological acidification in Xenopus oocyte.
Article in The Journal of membrane biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 14 citations in OpenAlex.
- Environmental and Endogenous Acids Can Trigger Allergic-Type Airway Reactions.International journal of environmental research and public health · 2020Review
- Adverse effects of fly ashes used as immobilizing agents for highly metal-contaminated soils on Xenopus laevis oocytes survival and maturation-a study performed in the north of France with field soil extracts.Environmental science and pollution research international · 2020Article
- Acid stress mediated adaptive divergence in ion channel function during embryogenesis in Rana arvalis.Scientific reports · 2015Article
- Nitric oxide-donor SNAP induces Xenopus eggs activation.PloS one · 2012Article
- Loss-of-function and gain-of-function phenotypes of stomatocytosis mutant RhAG F65S.American journal of physiology. Cell physiology · 2011Article
- Penetratin peptide potentiates endogenous calcium-activated chloride currents in Xenopus oocytes.The Journal of membrane biology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interplays between intracellular pH (pHi) and calcium ([Ca(2+)](i)) variations remain unclear, though both proton and calcium homeostasis changes accompany physiological events such as Xenopus laevis oocyte maturation. In this report, we used NH(4)Cl and changes of extracellular pH (pHe) to acidify the cytosol in a physiological range. In oocytes voltage-clamped at -80 mV, NH(4)Cl triggered an inward current, the main component of which is a Ca(2+)-dependent chloride current. Calcium imaging confirmed that NH(4)Cl provoked a [Ca(2+)](i) increase. The mobilized sources of calcium were discriminated using the triple-step protocol as a means to follow both the calcium-activated chloride currents (ICl-Ca) and the hyperpolarization- and acid-activated nonselective cation current (I(In)). These currents were stimulated during external addition of NH(4)Cl. This upregulation was abolished by BAPTA-AM, caffeine and heparin. By both buffering pHi changes with MOPS and by inhibiting calcium influx with lanthanum, intracellular acidification, initiated by NH(4)Cl and extracellular acidic medium, was shown to trigger a [Ca(2+)](i) increase through both calcium release and calcium influx. The calcium pathways triggered by pHe changes are similar to those activated by NH(4)Cl, thus suggesting that there is a robust signaling mechanism allowing the cell to adjust to variable environmental conditions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.