ArticleTraffic (Copenhagen, Denmark)2010
Trafficking and recycling of the connexin43 gap junction protein during mitosis.
Article in Traffic (Copenhagen, Denmark), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 59 citations in OpenAlex.
- Intercellular Communication via Mitotic Nanotubes is Influenced by Connexin-43 Trafficking and Actin Remodeling.bioRxiv : the preprint server for biology · 2026Article
- BECLIN-1/BECN1 at the barrier: a gatekeeper of epithelial and endothelial homeostasis.Autophagy reports · 2025Review
- Exploring temporal and sex-linked dysregulation in Alzheimer disease phosphoproteome.iScience · 2024Article
- Intercellular Communication Through Microtubular Highways.Results and problems in cell differentiation · 2024Review
- Regulation of gap junction intercellular communication by connexin ubiquitination: physiological and pathophysiological implications.Cellular and molecular life sciences : CMLS · 2020Review
- Early Optic Nerve Head Glial Proliferation and Jak-Stat Pathway Activation in Chronic Experimental Glaucoma.Investigative ophthalmology & visual science · 2019Article
- Visualization of Annular Gap Junction Vesicle Processing: The Interplay Between Annular Gap Junctions and Mitochondria.International journal of molecular sciences · 2018Article
- Dynamic intercellular transport modulates the spatial patterning of differentiation during early neural commitment.Nature communications · 2018Article
- Protein⁻Protein Interactions with Connexin 43: Regulation and Function.International journal of molecular sciences · 2018Review
- Connexins: Synthesis, Post-Translational Modifications, and Trafficking in Health and Disease.International journal of molecular sciences · 2018Review
- Distinct moieties underlie biphasic HFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Gap junction plasticity as a mechanism to regulate network-wide oscillations.PLoS computational biology · 2018Article
- Intracellular trafficking pathways of Cx43 gap junction channels.Biochimica et biophysica acta. Biomembranes · 2018Review
- Phosphorylation regulates connexin43/ZO-1 binding and release, an important step in gap junction turnover.Molecular biology of the cell · 2017Article
- Mitotic cells form actin-based bridges with adjacent cells to provide intercellular communication during rounding.Cell cycle (Georgetown, Tex.) · 2016Article
- The "tail" of Connexin43: An unexpected journey from alternative translation to trafficking.Biochimica et biophysica acta · 2016Review
- Connexin 43 and CaV1.2 Ion Channel Trafficking in Healthy and Diseased Myocardium.Circulation. Arrhythmia and electrophysiology · 2016Review
- Molecular mechanisms regulating formation, trafficking and processing of annular gap junctions.BMC cell biology · 2016Review
- Redistribution of connexin 43 during cell division.Cell biology international · 2016Article
- Dynamic changes in protein interaction between AKAP95 and Cx43 during cell cycle progression of A549 cells.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
During the cell cycle, gap junction communication, morphology and distribution of connexin43 (Cx43)-containing structures change dramatically. As cells round up in mitosis, Cx43 labeling is mostly intracellular and intercellular coupling is reduced. We investigated Cx43 distributions during mitosis both in endogenous and exogenous expressing cells using optical pulse-chase labeling, correlated light and electron microscopy, immunocytochemistry and biochemical analysis. Time-lapse imaging of green fluorescent protein (GFP)/tetracysteine tagged Cx43 (Cx43-GFP-4C) expressing cells revealed an early disappearance of gap junctions, progressive accumulation of Cx43 in cytoplasmic structures, and an unexpected subset pool of protein concentrated in the plasma membrane surrounding the midbody region in telophase followed by rapid reappearance of punctate plaques upon mitotic exit. These distributions were also observed in immuno-labeled endogenous Cx43-expressing cells. Photo-oxidation of ReAsH-labeled Cx43-GFP-4C cells in telophase confirmed that Cx43 is distributed in the plasma membrane surrounding the midbody as apparent connexons and in cytoplasmic vesicles. We performed optical pulse-chase labeling and single label time-lapse imaging of synchronized cells stably expressing Cx43 with internal tetracysteine domains through mitosis. In late telophase, older Cx43 is segregated mainly to the plasma membrane while newer Cx43 is intracellular. This older population nucleates new gap junctions permitting rapid resumption of communication upon mitotic exit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.