ArticleBioconjugate chemistry2010
Combinatorial approach to determine functional group effects on lipidoid-mediated siRNA delivery.
Article in Bioconjugate chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Rational design and modular synthesis of biodegradable ionizable lipids via the Passerini reaction for mRNA delivery.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Breaking the final barrier: Evolution of cationic and ionizable lipid structure in lipid nanoparticles to escape the endosome.Advanced drug delivery reviews · 2024Review
- A Comprehensive Review of mRNA Vaccines.International journal of molecular sciences · 2023Review
- Lipids and Lipid Derivatives for RNA Delivery.Chemical reviews · 2021Review
- High-throughput screening of nanoparticles in drug delivery.APL bioengineering · 2021Review
- Lipophilic Polyamines as Promising Components of Liposomal Gene Delivery Systems.Pharmaceutics · 2021Review
- Computational and Experimental Approaches to Investigate Lipid Nanoparticles as Drug and Gene Delivery Systems.Current topics in medicinal chemistry · 2021Review
- Intracellular Delivery of His-Tagged Genome-Editing Proteins Enabled by Nitrilotriacetic Acid-Containing Lipidoid Nanoparticles.Advanced healthcare materials · 2019Article
- Transfection by cationic gemini lipids and surfactants.MedChemComm · 2018Review
- Immunogenicity Testing of Lipidoids In Vitro and In Silico: Modulating Lipidoid-Mediated TLR4 Activation by Nanoparticle Design.Molecular therapy. Nucleic acids · 2018Article
- Inhibition of Hepatitis C Virus in Mice by a Small Interfering RNA Targeting a Highly Conserved Sequence in Viral IRES Pseudoknot.PloS one · 2016Article
- Guanabenz (Wytensin™) selectively enhances uptake and efficacy of hydrophobically modified siRNAs.Nucleic acids research · 2015Article
- Lipidoid Nanoparticles for siRNA Delivery to the Intestinal Epithelium: In Vitro Investigations in a Caco-2 Model.PloS one · 2015Article
- Liposome-like Nanostructures for Drug Delivery.Journal of materials chemistry. B · 2013Article
- Delivery materials for siRNA therapeutics.Nature materials · 2013Review
- Article
- Lipid-modified aminoglycoside derivatives for in vivo siRNA delivery.Advanced materials (Deerfield Beach, Fla.) · 2013Article
- Biodegradable lipids enabling rapidly eliminated lipid nanoparticles for systemic delivery of RNAi therapeutics.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Redox-based control of the transformation and activation of siRNA complexes in extracellular environments using ferrocenyl lipids.Journal of the American Chemical Society · 2013Article
- Neutral polymeric micelles for RNA delivery.Bioconjugate chemistry · 2013Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
The application of RNA interference (RNAi), either in the clinic or in the laboratory, requires safe and effective delivery methods. Here, we develop a combinatorial approach to synthesize a library of delivery vectors based on two lipid-like substrates with known siRNA delivery capabilities. Members of this library have a mixture of lipid-like tails and feature appendages containing hydroxyl, carbamate, ether, or amine functional groups as well as variations in alkyl chain length and branching. Using a luciferase reporter system in HeLa cells, we studied the relationship between lipid chemical modification and delivery performance in vitro. The impact of the functional group was shown to vary depending on the overall amine content and tail number of the delivery vector. Additionally, in vivo performance was evaluated using a Factor VII knockdown assay. Two library members, each containing ether groups, were found to knock down the target protein at levels comparable to those of the parent delivery vector. These results demonstrate that small chemical changes to the delivery vector impact knockdown efficiency and cell viability both in vitro and in vivo. The work described here identifies new materials for siRNA delivery and provides new insight into the parameters for optimized chemical makeup of lipid-like siRNA delivery materials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.