ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2011
Real-time measurement of solute transport within the lacunar-canalicular system of mechanically loaded bone: direct evidence for load-induced fluid flow.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 112 papers.
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Who cites it
112 citing papers in PubMed.
- Mechanical environment change in root, periodontal ligament, and alveolar bone in response to two canine retraction treatment strategies.Orthodontics & craniofacial research · 2015Trial
- Short-term bone formation is greatest within high strain regions of the human distal radius: a prospective pilot study.Journal of biomechanical engineering · 2015Trial
- Divergent trajectories of structural accrual and matrix-level mechanical properties during alveolar bone maturation.Calcified tissue international · 2026Article
- Intravital Depletion of Cholesterol Reduces Membrane Dynamics and Increases Mechanosensitivity in Osteocytes In Vivo.bioRxiv : the preprint server for biology · 2026Article
- Permeability of bone and cartilage, and stiffness of collagen within cartilage, influence osteochondral fluid transport during cyclic compression: A study in finite elements.Journal of the mechanical behavior of biomedical materials · 2026Article
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- Integrating 3D Osteocyte Culture, Microgravity Simulation, and Fluid Flow Reveals Mechanisms of Osteocyte Mechanosensation and Calcium Signaling Altered by Disuse.Biomolecules · 2025Article
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- Predicting cortical bone resorption in the mouse tibia under disuse conditions caused by transient muscle paralysis.Scientific reports · 2025Article
- Increased deformations are dispensable for encapsulated cell mechanoresponse in engineered bone analogs mimicking aging bone marrow.Mechanobiology in medicine · 2025Article
- Numerical study of interstitial fluid flow behavior in osteons under dynamic loading.BMC musculoskeletal disorders · 2025Article
- Involvement of long non-coding RNA (lncRNA) MALAT1 in shear stress regulated adipocyte differentiation.Frontiers in bioengineering and biotechnology · 2025Article
- Isolation, Purification, Generation, and Culture of Osteocytes.Methods in molecular biology (Clifton, N.J.) · 2025Article
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- Evidence for peri-lacunar remodeling and altered osteocyte lacuno-canalicular network in mouse models of myeloma-induced bone disease.JBMR plus · 2024Article
- Prkd1 regulates the formation and repair of plasma membrane disruptions (PMD) in osteocytes.Bone · 2024Article
- Mechanobiology and Primary Cilium in the Pathophysiology of Bone Marrow Myeloproliferative Diseases.International journal of molecular sciences · 2024Review
- The roles of extracellular vesicles released by mechanically stimulated osteocytes in regulating osteoblast and osteoclast functions.Mechanobiology in medicine · 2024Article
- A Novel Primary Cilium-Mediated Mechanism Through which Osteocytes Regulate Metastatic Behavior of Both Breast and Prostate Cancer Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
52 more citing papers are in PubMed but not listed here.
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4 authors.
Funding
Abstract
Since proposed by Piekarski and Munro in 1977, load-induced fluid flow through the bone lacunar-canalicular system (LCS) has been accepted as critical for bone metabolism, mechanotransduction, and adaptation. However, direct unequivocal observation and quantification of load-induced fluid and solute convection through the LCS have been lacking due to technical difficulties. Using a novel experimental approach based on fluorescence recovery after photobleaching (FRAP) and synchronized mechanical loading and imaging, we successfully quantified the diffusive and convective transport of a small fluorescent tracer (sodium fluorescein, 376 Da) in the bone LCS of adult male C57BL/6J mice. We demonstrated that cyclic end-compression of the mouse tibia with a moderate loading magnitude (-3 N peak load or 400 µε surface strain at 0.5 Hz) and a 4-second rest/imaging window inserted between adjacent load cycles significantly enhanced (+31%) the transport of sodium fluorescein through the LCS compared with diffusion alone. Using an anatomically based three-compartment transport model, the peak canalicular fluid velocity in the loaded bone was predicted (60 µm/s), and the resulting peak shear stress at the osteocyte process membrane was estimated (∼5 Pa). This study convincingly demonstrated the presence of load-induced convection in mechanically loaded bone. The combined experimental and mathematical approach presented herein represents an important advance in quantifying the microfluidic environment experienced by osteocytes in situ and provides a foundation for further studying the mechanisms by which mechanical stimulation modulates osteocytic cellular responses, which will inform basic bone biology, clinical understanding of osteoporosis and bone loss, and the rational engineering of their treatments.
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