ArticlePLoS pathogens2010
Bacteriophage lysin mediates the binding of streptococcus mitis to human platelets through interaction with fibrinogen.
Article in PLoS pathogens, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 61 citations in OpenAlex.
- Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release.Scientific reports · 2016Trial
- Serotype-Dependent Inhibition ofJournal of microbiology and biotechnology · 2024Article
- The Burden of Survivors: How Can Phage Infection Impact Non-Infected Bacteria?International journal of molecular sciences · 2023Review
- The microbiome-product colibactin hits unique cellular targets mediating host-microbe interaction.Frontiers in pharmacology · 2022Review
- Atypical Bacilliredoxin AbxC Plays a Role in Responding to Oxidative Stress in Radiation-Resistant BacteriumAntioxidants (Basel, Switzerland) · 2021Article
- Complete Genome Sequence of Streptococcus oralis SF100, Isolated from Blood Cultures from a Patient with Infective Endocarditis.Microbiology resource announcements · 2021Article
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- Induction of Apoptotic Cell Death by Oral Streptococci in Human Periodontal Ligament Cells.Frontiers in microbiology · 2021Article
- A Champion of Host Defense: A Generic Large-Scale Cause for Platelet Dysfunction and Depletion in Infection.Seminars in thrombosis and hemostasis · 2020Review
- Genomic and Proteomic Characterization of Bacteriophage BH1 Spontaneously Released from ProbioticViruses · 2019Article
- Recognition of specific sialoglycan structures by oral streptococci impacts the severity of endocardial infection.PLoS pathogens · 2019Article
- Serotype-Independent Protection Against Invasive Pneumococcal Infections Conferred by Live Vaccine WithFrontiers in immunology · 2019Article
- Gamma-irradiation of Streptococcus pneumoniae for the use as an immunogenic whole cell vaccine.Journal of microbiology (Seoul, Korea) · 2018Article
- Molecular characterization of pneumococcal surface protein K, a potential pneumococcal vaccine antigen.Virulence · 2017Article
- Perturbations of Phosphatidate Cytidylyltransferase (CdsA) Mediate Daptomycin Resistance in Streptococcus mitis/oralis by a Novel Mechanism.Antimicrobial agents and chemotherapy · 2017Article
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- The Pil3 pilus of Streptococcus gallolyticus binds to intestinal mucins and to fibrinogen.Gut microbes · 2016Article
- The role of Staphylococcal carotenogenesis in resistance to host defense peptides and in vivo virulence in experimental endocarditis model.Pathogens and disease · 2015Article
- A novel adhesive factor contributing to the virulence of Vibrio parahaemolyticus.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
The binding of bacteria to human platelets is a likely central mechanism in the pathogenesis of infective endocarditis. We have previously found that platelet binding by Streptococcus mitis SF100 is mediated by surface components encoded by a lysogenic bacteriophage, SM1. We now demonstrate that SM1-encoded lysin contributes to platelet binding via its direct interaction with fibrinogen. Far Western blotting of platelets revealed that fibrinogen was the major membrane-associated protein bound by lysin. Analysis of lysin binding with purified fibrinogen in vitro confirmed that these proteins could bind directly, and that this interaction was both saturable and inhibitable. Lysin bound both the Aalpha and Bbeta chains of fibrinogen, but not the gamma subunit. Binding of lysin to the Bbeta chain was further localized to a region within the fibrinogen D fragment. Disruption of the SF100 lysin gene resulted in an 83+/-3.1% reduction (mean +/- SD) in binding to immobilized fibrinogen by this mutant strain (PS1006). Preincubation of this isogenic mutant with purified lysin restored fibrinogen binding to wild type levels. When tested in a co-infection model of endocarditis, loss of lysin expression resulted in a significant reduction in virulence, as measured by achievable bacterial densities (CFU/g) within vegetations, kidneys, and spleens. These results indicate that bacteriophage-encoded lysin is a multifunctional protein, representing a new class of fibrinogen-binding proteins. Lysin appears to be cell wall-associated through its interaction with choline. Once on the bacterial surface, lysin can bind fibrinogen directly, which appears to be an important interaction for the pathogenesis of endocarditis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.