ArticleGenes & development2010
Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud.
Article in Genes & development, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 82 citations in OpenAlex.
- Cellular imbalance of specific RNA-binding proteins associates with harmful R-loops.PLoS genetics · 2025Article
- NusG-Spt5 Transcription Factors: Universal, Dynamic Modulators of Gene Expression.Journal of molecular biology · 2025Review
- Phosphorylation controls the oligomeric state of She2 and mRNA localization in yeast.RNA (New York, N.Y.) · 2023Article
- RNA transport from transcription to localized translation: a single molecule perspective.RNA biology · 2021Review
- Messenger RNA transport in the opportunistic fungal pathogen Candida albicans.Current genetics · 2017Review
- Binding of DEAD-box helicase Dhh1 to the 5'-untranslated region ofThe Journal of biological chemistry · 2017Article
- Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex.Nature structural & molecular biology · 2017Article
- The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein.Protein science : a publication of the Protein Society · 2016Article
- Whisper mutations: cryptic messages within the genetic code.Oncogene · 2016Review
- ASH1 mRNP-core factors form stable complexes in absence of cargo RNA at physiological conditions.RNA biology · 2015Article
- In Candida albicans hyphae, Sec2p is physically associated with SEC2 mRNA on secretory vesicles.Molecular microbiology · 2014Article
- Nervous translation, do you get the message? A review of mRNPs, mRNA-protein interactions and translational control within cells of the nervous system.Cellular and molecular life sciences : CMLS · 2014Review
- Co-transcriptional recruitment of Puf6 by She2 couples translational repression to mRNA localization.Nucleic acids research · 2014Article
- Structure of a myosin•adaptor complex and pairing by cargo.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- DNA dynamics during early double-strand break processing revealed by non-intrusive imaging of living cells.PLoS genetics · 2014Article
- Coupling mRNA processing with transcription in time and space.Nature reviews. Genetics · 2014Review
- Here, there, everywhere. mRNA localization in budding yeast.RNA biology · 2014Review
- Of social molecules: The interactive assembly of ASH1 mRNA-transport complexes in yeast.RNA biology · 2014Review
- Role of Loc1p in assembly and reorganization of nuclear ASH1 messenger ribonucleoprotein particles in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- In vitro reconstitution of an mRNA-transport complex reveals mechanisms of assembly and motor activation.The Journal of cell biology · 2013Article
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3 authors at 1 institution in 1 country.
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Abstract
Pre-mRNA processing is coupled with transcription. It is still unclear if the transcription machinery can also directly affect the cytoplasmic fate of a transcript, such as its intracellular localization. In yeast, the RNA-binding protein She2p binds several mRNAs and targets them for localization at the bud. Here we report that She2p is recruited cotranscriptionally to the nascent bud-localized ASH1, IST2, and EAR1 mRNA. She2p interacts in vivo with the elongating forms of RNA polymerase II (pol II) via the transcription elongation factor Spt4-Spt5. Mutations in either SPT4 or SPT5 reduce the cotranscriptional recruitment of She2p on the ASH1 gene, disrupt the proper localization of ASH1 mRNA at the bud tip, and affect Ash1p sorting to the daughter cell nucleus. We propose that She2p is recruited by the RNA pol II machinery prior to its transfer to nascent bud-localized mRNAs. Indeed, She2p is present with RNA pol II on genes coding for localized or nonlocalized transcripts, but is associated with nascent mRNA only on genes coding for bud-localized transcripts. Moreover, a She2p mutant defective in RNA binding still associates with RNA pol II transcribed genes. This study uncovers a novel mechanism for the cotranscriptional assembly of mRNP complexes primed for localization in the cytoplasm.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.