ArticleBiophysical journal2010
Microfiberoptic measurement of extracellular space volume in brain and tumor slices based on fluorescent dye partitioning.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
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- Brain clearance is reduced during sleep and anesthesia.Nature neuroscience · 2024Article
- Generating immunogenomic data-guided virtual patients using a QSP model to predict response of advanced NSCLC to PD-L1 inhibition.NPJ precision oncology · 2023Article
- Generating immunogenomic data-guided virtual patients using a QSP model to predict response of advanced NSCLC to PD-L1 inhibition.bioRxiv : the preprint server for biology · 2023Article
- Non-Transport Functions of Aquaporins.Advances in experimental medicine and biology · 2023Article
- Prediction of Lung Shunt Fraction for Yttrium-90 Treatment of Hepatic Tumors Using Dynamic Contrast Enhanced MRI with Quantitative Perfusion Processing.Tomography (Ann Arbor, Mich.) · 2022Article
- Intercellular Communication in the Central Nervous System as Deduced by Chemical Neuroanatomy and Quantitative Analysis of Images: Impact on Neuropharmacology.International journal of molecular sciences · 2022Review
- Conductance of porous media depends on external electric fields.Biophysical journal · 2021Article
- Characterizing extracellular diffusion properties using diffusion-weighted MRS of sucrose injected in mouse brain.NMR in biomedicine · 2021Article
- Spatial Organization and Dynamics of the Extracellular Space in the Mouse Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Current Techniques for Investigating the Brain Extracellular Space.Frontiers in neuroscience · 2020Review
- Longitudinal evaluation of tumor microenvironment in rat focal brainstem glioma using diffusion and perfusion MRI.Journal of magnetic resonance imaging : JMRI · 2019Article
- Early changes in the apparent diffusion coefficient and MMP-9 expression of a cervical carcinoma U14 allograft model following irradiation.Oncology letters · 2017Article
- The aquaporin-4 water channel as a potential drug target in neurological disorders.Expert opinion on therapeutic targets · 2017Review
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- Nanoscale diffusion in the synaptic cleft and beyond measured with time-resolved fluorescence anisotropy imaging.Scientific reports · 2017Article
- Radionuclide I-131 Labeled Albumin-Paclitaxel Nanoparticles for Synergistic Combined Chemo-radioisotope Therapy of Cancer.Theranostics · 2017Article
- A pre-tracer approach for improving the accuracy of metabolic measurements by hyperpolarized nuclear magnetic resonance.Quantitative imaging in medicine and surgery · 2016Article
- Stromal uptake and transmission of acid is a pathway for venting cancer cell-generated acid.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Sensory-Evoked Intrinsic Imaging Signals in the Olfactory Bulb Are Independent of Neurovascular Coupling.Cell reports · 2015Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The fractional volume occupied by extracellular space in tissues, termed alpha, is an important parameter of tissue architecture that affects cellular functions and drug delivery. We report a technically simple fluorescent dye partitioning method to measure alpha in tissue slices based on microfiberoptic detection of dye fluorescence in tissue versus overlying solution. Microfiberoptic tip geometry and dyes were selected for alpha determination from fluorescence intensity ratios, without the need to correct for illumination profile, light scattering/absorption, or dye binding. The method was validated experimentally using cell-embedded gels of specified alpha-values and optical properties. In mouse brain slices, alpha was strongly location-dependent, ranging from 0.16 in thalamus to 0.22 in brainstem, and was sensitive to cell volume changes. Aquaporin-4 water channel gene deletion caused significant extracellular space expansion, with alpha = 0.181 +/- 0.002 in cortex in wild-type mice and 0.211 +/- 0.003 in Aquaporin-4 knockout mice. In slices of LLC1 cell tumors grown in mice to approximately 5 mm diameter, alpha decreased remarkably from approximately 0.45 in superficial tumor to <0.25 in deeper (>100 mum) tumor. Fluorescent dye partitioning with microfiberoptic detection permits rapid, accurate, and anisotropy-insensitive determination of alpha-values in tissue slices.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.