ArticleBiophysical journal2010
Cholesterol displaces palmitoylceramide from its tight packing with palmitoylsphingomyelin in the absence of a liquid-disordered phase.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 42 citations in OpenAlex.
- Inimitable Impacts of Ceramides on Lipid Rafts Formed in Artificial and Natural Cell Membranes.Membranes · 2022Review
- Influence of ceramide on lipid domain stability studied with small-angle neutron scattering: The role of acyl chain length and unsaturation.Chemistry and physics of lipids · 2022Article
- Sphingomyelinase-Mediated Multitimescale Clustering of Ganglioside GM1 in Heterogeneous Lipid Membranes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Lamellar Phases Composed of Phospholipid, Cholesterol, and Ceramide, as Studied byBiophysical journal · 2019Article
- Cholesterol-pyrene as a probe for cholesterol distribution on ordered and disordered membranes: Determination of spectral wavelengths.PloS one · 2018Article
- The many faces (and phases) of ceramide and sphingomyelin II - binary mixtures.Biophysical reviews · 2017Review
- Ceramide-C16 Is a Versatile Modulator of Phosphatidylethanolamine Polymorphism.Biophysical journal · 2017Article
- Bilayer Interactions among Unsaturated Phospholipids, Sterols, and Ceramide.Biophysical journal · 2017Article
- The Long-Chain Sphingoid Base of Ceramides Determines Their Propensity for Lateral Segregation.Biophysical journal · 2017Article
- Influence of Hydroxylation, Chain Length, and Chain Unsaturation on Bilayer Properties of Ceramides.Biophysical journal · 2015Article
- Lamellar gel (lβ) phases of ternary lipid composition containing ceramide and cholesterol.Biophysical journal · 2014Article
- Micrometric segregation of fluorescent membrane lipids: relevance for endogenous lipids and biogenesis in erythrocytes.Journal of lipid research · 2013Article
- Insights into sphingolipid miscibility: separate observation of sphingomyelin and ceramide N-acyl chain melting.Biophysical journal · 2012Article
- In situ synthesis of fluorescent membrane lipids (ceramides) using click chemistry.Journal of chemical biology · 2012Article
- Mixing properties of sphingomyelin ceramide bilayers: a simulation study.The journal of physical chemistry. B · 2012Article
- Effects of sphingosine 2N- and 3O-methylation on palmitoyl ceramide properties in bilayer membranes.Biophysical journal · 2011Article
- Organization and dynamics of Fas transmembrane domain in raft membranes and modulation by ceramide.Biophysical journal · 2011Article
- The effects of N-acyl chain methylations on ceramide molecular properties in bilayer membranes.European biophysics journal : EBJ · 2011Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A set of different biophysical approaches has been used to explore the phase behavior of palmitoylsphingomyelin (pSM)/cholesterol (Chol) model membranes in the presence and absence of palmitoylceramide (pCer). Fluorescence spectroscopy of di-4-ANEPPDHQ-stained pSM/Chol vesicles and atomic force microscopy of supported planar bilayers show gel L(beta)/liquid-ordered (L(o)) phase coexistence within the range X(Chol) = 0-0.25 at 22 degrees C. At the latter compositional point and beyond, a single L(o) pSM/Chol phase is detected. In ternary pSM/Chol/pCer mixtures, differential scanning calorimetry of multilamellar vesicles and confocal fluorescence microscopy of giant unilamellar vesicles concur in showing immiscibility, but no displacement, between L(o) cholesterol-enriched (pSM/Chol) and gel-like ceramide-enriched (pSM/pCer) phases at high pSM/(Chol + pCer) ratios. At higher cholesterol content, pCer is unable to displace cholesterol at any extent, even at X(Chol) < 0.25. It is interesting that an opposite strong cholesterol-mediated pCer displacement from its tight packing with pSM is clearly detected, completely abolishing the pCer ability to generate large microdomains and giving rise instead to a single ternary phase. These observations in model membranes in the absence of the lipids commonly used to form a liquid-disordered phase support the role of cholesterol as the key determinant in controlling its own displacement from L(o) domains by ceramide upon sphingomyelinase activity.
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