ArticleBiophysical journal2010
High mobility of bicoid captured by fluorescence correlation spectroscopy: implication for the rapid establishment of its gradient.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 114 citations in OpenAlex.
- Dendritic Membranized Coacervate Microdroplets: A Robust Platform for Synthetic-Living Cell Consortia.Journal of the American Chemical Society · 2025Article
- Both the transcriptional activator, Bcd, and repressor, Cic, form small mobile oligomeric clusters.Biophysical journal · 2025Article
- Transcription factor clusters as information transfer agents.Science advances · 2025Article
- Stable developmental patterns of gene expression without morphogen gradients.PLoS computational biology · 2024Article
- Article
- 4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector.Nature communications · 2024Article
- Deep learning reduces data requirements and allows real-time measurements in imaging FCS.Biophysical journal · 2024Article
- Long-range formation of the Bicoid gradient requires multiple dynamic modes that spatially vary across the embryo.Development (Cambridge, England) · 2024Article
- Revisiting bicoid function: complete inactivation reveals an additional fundamental role in Drosophila egg geometry specification.Hereditas · 2024Article
- Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications.Communications biology · 2023Review
- How does an organism extract relevant information from transcription factor concentrations?Biochemical Society transactions · 2022Review
- Patterning principles of morphogen gradients.Open biology · 2022Review
- Synthetic reconstruction of theeLife · 2022Article
- Trading bits in the readout from a genetic network.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Using FCS to accurately measure protein concentration in the presence of noise and photobleaching.Biophysical journal · 2021Article
- High rates of calcium-free diffusion in the cytosol of living cells.Biophysical journal · 2021Article
- Capicua is a fast-acting transcriptional brake.Current biology : CB · 2021Article
- Modulating the bicoid gradient in space and time.Hereditas · 2021Article
- Of numbers and movement - understanding transcription factor pathogenesis by advanced microscopy.Disease models & mechanisms · 2020Review
- Phase separation at the nanoscale quantified by dcFCCS.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Bicoid (Bcd) morphogen is essential for pattern formation in fruit flies. It forms an exponential concentration gradient along the embryo AP axis and turns on cascades of target genes in distinct anterior domains. The most commonly accepted model for gradient formation assumes that Bcd travels by simple diffusion and is uniformly degraded across syncytial embryos, yet several recent studies have challenged these ideas. Here, the question of Bcd mobility was investigated using fluorescence correlation spectroscopy in live Drosophila melanogaster embryos. Bcd-EGFP molecules were found to be highly mobile in the cytoplasm during cycles 12-14, with a diffusion coefficient approximately 7 microm(2)/s. This value is large enough to explain the stable establishment of the Bcd gradient simply by diffusion before cycle 8, i.e., before the onset of zygotic transcription.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.