ArticleJournal of the American Chemical Society2010
High-fidelity hydrophilic probe for two-photon fluorescence lysosomal imaging.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 172 citations in OpenAlex.
- pH-Sensitive and Lysosome Targetable Photosensitizers Based on BODIPYs.Journal of fluorescence · 2025Article
- Near-Infrared Bioimaging Using Two-photon Fluorescent Probes.Advanced healthcare materials · 2025Review
- Molecular Fluorophores for Deep-Tissue Bioimaging.ACS central science · 2020Review
- Tuning the π-bridge of quadrupolar triarylborane chromophores for one- and two-photon excited fluorescence imaging of lysosomes in live cells.Chemical science · 2019Article
- Through-Bond Energy Transfer Cassette with Dual-Stokes Shifts for "Double Checked" Cell Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Article
- Fluorenyl-Loaded Quatsome Nanostructured Fluorescent Probes.ACS omega · 2017Article
- Synthesis of Near-Infrared Fluorescent Two-Photon-Absorbing Fluorenyl Benzothiadiazole and Benzoselenadiazole Derivatives.ACS omega · 2016Article
- Nucleic acid-selective light-up fluorescent biosensors for ratiometric two-photon imaging of the viscosity of live cells and tissues.Chemical science · 2016Article
- A ratiometric two-photon probe for quantitative imaging of mitochondrial pH values.Chemical science · 2016Article
- Lysosomal targeting with stable and sensitive fluorescent probes (Superior LysoProbes): applications for lysosome labeling and tracking during apoptosis.Scientific reports · 2015Article
- Highly stable and sensitive fluorescent probes (LysoProbes) for lysosomal labeling and tracking.Scientific reports · 2015Article
- A fluorescent indicator for imaging lysosomal zinc(II) with Förster resonance energy transfer (FRET)-enhanced photostability and a narrow band of emission.Chemistry (Weinheim an der Bergstrasse, Germany) · 2015Article
- Upconversion luminescence imaging of cells and small animals.Nature protocols · 2013Article
- Selective cell death by photochemically induced pH imbalance in cancer cells.Journal of the American Chemical Society · 2013Article
- Small molecule fluorophore and copolymer RGD peptide conjugates for ex vivo two-photon fluorescence tumor vasculature imaging.Biomaterials · 2012Article
- Two-photon absorption and time-resolved stimulated emission depletion spectroscopy of a new fluorenyl derivative.Chemphyschem : a European journal of chemical physics and physical chemistry · 2012Article
- Near-infrared-emitting squaraine dyes with high 2PA cross-sections for multiphoton fluorescence imaging.ACS applied materials & interfaces · 2012Article
- Transient excited-state absorption and gain spectroscopy of a two-photon absorbing probe with efficient superfluorescent properties.The journal of physical chemistry. C, Nanomaterials and interfaces · 2012Article
- Two-photon fluorescence microscopy imaging of cellular oxidative stress using profluorescent nitroxides.Journal of the American Chemical Society · 2012Article
- Two-photon STED spectral determination for a new V-shaped organic fluorescent probe with efficient two-photon absorption.Chemphyschem : a European journal of chemical physics and physical chemistry · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The synthesis and characterization of a novel two-photon-absorbing fluorene derivative, LT1, selective for the lysosomes of HCT 116 cancer cells, is reported. Linear and nonlinear photophysical and photochemical properties of the probe were investigated to evaluate the potential of the probe for two-photon fluorescence microscopy (2PFM) lysosomal imaging. The cytotoxicity of the probe was investigated to evaluate the potential of using this probe for live two-photon fluorescence biological imaging applications. Colocalization studies of the probe with commercial Lysotracker Red in HCT 116 cells demonstrated the specific localization of the probe in the lysosomes with an extremely high colocalization coefficient (0.96). A figure of merit was introduced to allow comparison between probes. LT1 has a number of properties that far exceed those of commercial lysotracker probes, including higher two-photon absorption cross sections, good fluorescence quantum yield, and, importantly, high photostability, all resulting in a superior figure of merit. 2PFM was used to demonstrate lysosomal tracking with LT1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.