ArticleHistochemistry and cell biology2010
Development of image analysis tool for the classification of muscle fibre type using immunohistochemical staining.
Article in Histochemistry and cell biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Characterization of Fiber-Type Composition and Phosphoproteins of Fast- and Slow-Growing Broilers.Animals : an open access journal from MDPI · 2026Article
- An alternative mechanism for skeletal muscle dysfunction in long-term post-viral lung disease.American journal of physiology. Lung cellular and molecular physiology · 2023Article
- Influence of Dietary Protein Source and Level on Histological Properties of Muscle and Adipose Tissue of Lambs.Foods (Basel, Switzerland) · 2023Article
- A Single Bout of Ultra-Endurance Exercise Reveals Early Signs of Muscle Aging in Master Athletes.International journal of molecular sciences · 2022Article
- Approach for semi-automated measurement of fiber diameter in murine and canine skeletal muscle.PloS one · 2020Article
- Relationships Between Cull Beef Cow Characteristics, Finishing Practices and Meat Quality Traits ofFoods (Basel, Switzerland) · 2019Article
- Study of the Chronology of Expression of Ten Extracellular Matrix Molecules during the Myogenesis in Cattle to Better Understand Sensory Properties of Meat.Foods (Basel, Switzerland) · 2019Article
- MyoVision: software for automated high-content analysis of skeletal muscle immunohistochemistry.Journal of applied physiology (Bethesda, Md. : 1985) · 2018Article
- Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition.Journal of visualized experiments : JoVE · 2017Article
- A novel triple immunoenzyme staining enables simultaneous identification of all muscle fiber types on a single skeletal muscle cryosection from normal, denervated or reinnervated rats.Neural regeneration research · 2017Article
- Colonisation of Meat byFrontiers in microbiology · 2017Article
- Impact of TIEG1 Deletion on the Passive Mechanical Properties of Fast and Slow Twitch Skeletal Muscles in Female Mice.PloS one · 2016Article
- The Invalidation of HspB1 Gene in Mouse Alters the Ultrastructural Phenotype of Muscles.PloS one · 2016Article
- How Muscle Structure and Composition Influence Meat and Flesh Quality.TheScientificWorldJournal · 2016Review
- A simplified immunohistochemical classification of skeletal muscle fibres in mouse.European journal of histochemistry : EJH · 2014Article
- Quantifiable diagnosis of muscular dystrophies and neurogenic atrophies through network analysis.BMC medicine · 2013Article
- Histochemistry and cell biology: the annual review 2010.Histochemistry and cell biology · 2011Review
- Structural and functional alterations of the cell nucleus in skeletal muscle wasting: the evidence in situ.European journal of histochemistry : EJH · 2010Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An accurate characterisation of muscle fibres is essential for studying muscle plasticity. During some transient events such as ageing, myogenesis, physical activity or conversion of muscle to meat, the morphological parameters and/or the fibre type distribution may change. Nowadays, this information is generally obtained using immunohistology techniques, but these analyses are acknowledged to be laborious and time-consuming. In fact, each myofibre, from thousands, must be measured individually and its expression profile in response to different anti-myosin antibodies must be established step by step. In this paper, we describe a new histological approach using double-labelling (laminin, myosin) serial sections, fluorescence microscopy visualisation and, finally, semi-automatic image analysis. The goal of the study was to propose a tool allowing faster fibre type characterisation, including the identification of hybrid fibres from pure ones. The steps in the image processing prone to subjectivity have been fully automated. On the other hand, the expert retained control of all image analysis procedures requiring visual diagnosis. The tool that we developed with the Visilog software allowed a rapid and objective fibre typing and morphometric characterisation of two different bovine muscles. The results were in agreement with our previous histological and densitometric assays. The method and the tool proved to be potentially more efficient than other techniques used in our institute or described in the literature. A more global evaluation will be considered in other laboratories as well as on other animal species.
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Registered trials
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