ArticleMolecular bioSystems2010
The emerging process of Top Down mass spectrometry for protein analysis: biomarkers, protein-therapeutics, and achieving high throughput.
Article in Molecular bioSystems, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Intact quantitative bioanalytical method development and fit-for-purpose validation of a monoclonal antibody and its related fab fragment in human vitreous and aqueous humor using LC-HRMS.Analytical and bioanalytical chemistry · 2022Article
- Enhanced Top-Down Protein Characterization with Electron Capture Dissociation and Cyclic Ion Mobility Spectrometry.Analytical chemistry · 2022Article
- Harnessing microbial wealth for lignocellulose biomass valorization through secretomics: a review.Biotechnology for biofuels · 2021Review
- Recent trends of capillary electrophoresis-mass spectrometry in proteomics research.Mass spectrometry reviews · 2019Review
- Combined Mass Spectrometry Imaging and Top-down Microproteomics Reveals Evidence of a Hidden Proteome in Ovarian Cancer.EBioMedicine · 2017Article
- De Novo Sequencing of Top-Down Tandem Mass Spectra: A Next Step towards Retrieving a Complete Protein Sequence.Proteomes · 2017Article
- Top-down analysis of protein samples by de novo sequencing techniques.Bioinformatics (Oxford, England) · 2016Article
- Enhanced Dissociation of Intact Proteins with High Capacity Electron Transfer Dissociation.Journal of the American Society for Mass Spectrometry · 2016Article
- Top Down Proteomics Reveals Mature Proteoforms Expressed in Subcellular Fractions of the Echinococcus granulosus Preadult Stage.Journal of proteome research · 2015Article
- Quantitative measurement of intact alpha-synuclein proteoforms from post-mortem control and Parkinson's disease brain tissue by intact protein mass spectrometry.Scientific reports · 2014Article
- Review
- Photodissociation mass spectrometry: new tools for characterization of biological molecules.Chemical Society reviews · 2014Review
- Top Down proteomics: facts and perspectives.Biochemical and biophysical research communications · 2014Review
- Fragmentation follows structure: top-down mass spectrometry elucidates the topology of engineered cystine-knot miniproteins.PloS one · 2014Article
- Spectral probabilities of top-down tandem mass spectra.BMC genomics · 2014Article
- Article
- Developing top down proteomics to maximize proteome and sequence coverage from cells and tissues.Current opinion in chemical biology · 2013Review
- Post-translational modification by cysteine protects Cu/Zn-superoxide dismutase from oxidative damage.Biochemistry · 2013Article
- Top-down MS for rapid methionine oxidation site assignment in filgrastim.Analytical and bioanalytical chemistry · 2013Article
- Spatially-directed protein identification from tissue sections by top-down LC-MS/MS with electron transfer dissociation.Analytical chemistry · 2013Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Top Down mass spectrometry (MS) has emerged as an alternative to common Bottom Up strategies for protein analysis. In the Top Down approach, intact proteins are fragmented directly in the mass spectrometer to achieve both protein identification and characterization, even capturing information on combinatorial post-translational modifications. Just in the past two years, Top Down MS has seen incremental advances in instrumentation and dedicated software, and has also experienced a major boost from refined separations of whole proteins in complex mixtures that have both high recovery and reproducibility. Combined with steadily advancing commercial MS instrumentation and data processing, a high-throughput workflow covering intact proteins and polypeptides up to 70 kDa is directly visible in the near future.
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Registered trials
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