ArticlePloS one2010
Targeting of embryonic stem cells by peptide-conjugated quantum dots.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Photosensitive nanocarriers for specific delivery of cargo into cells.Scientific reports · 2020Article
- Virus-Derived Peptides for Clinical Applications.Chemical reviews · 2017Review
- A scFv antibody targeting common oligomeric epitope has potential for treating several amyloidoses.Scientific reports · 2016Article
- Combinatorial peptide libraries: mining for cell-binding peptides.Chemical reviews · 2014Review
- CLAVATA3 dodecapeptide modified CdTe nanoparticles: a biocompatible quantum dot probe for in vivo labeling of plant stem cells.PloS one · 2014Article
- Targeting human embryonic stem cells with quantum dot-conjugated phages.Scientific reports · 2013Article
- Stem cell tracking with optically active nanoparticles.American journal of nuclear medicine and molecular imaging · 2013Article
- Identification of human embryonic progenitor cell targeting peptides using phage display.PloS one · 2013Article
- Phage display--a powerful technique for immunotherapy: 1. Introduction and potential of therapeutic applications.Human vaccines & immunotherapeutics · 2012Review
- Potential role of stem cells in severe spinal cord injury: current perspectives and clinical data.Stem cells and cloning : advances and applications · 2012Review
- Embryonic stem cell markers.Molecules (Basel, Switzerland) · 2012Review
- Cellular therapies for treating pain associated with spinal cord injury.Journal of translational medicine · 2012Review
- Quantum dots do not affect the behaviour of mouse embryonic stem cells and kidney stem cells and are suitable for short-term tracking.PloS one · 2012Article
- Diversity of phage-displayed libraries of peptides during panning and amplification.Molecules (Basel, Switzerland) · 2011Review
- Phage display: selecting straws instead of a needle from a haystack.Molecules (Basel, Switzerland) · 2011Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTargeting stem cells holds great potential for studying the embryonic stem cell and development of stem cell-based regenerative medicine. Previous studies demonstrated that nanoparticles can serve as a robust platform for gene delivery, non-invasive cell imaging, and manipulation of stem cell differentiation. However specific targeting of embryonic stem cells by peptide-linked nanoparticles has not been reported. METHODOLOGY/PRINCIPAL
findingsHere, we developed a method for screening peptides that specifically recognize rhesus macaque embryonic stem cells by phage display and used the peptides to facilitate quantum dot targeting of embryonic stem cells. Through a phage display screen, we found phages that displayed an APWHLSSQYSRT peptide showed high affinity and specificity to undifferentiated primate embryonic stem cells in an enzyme-linked immunoabsorbent assay. These results were subsequently confirmed by immunofluorescence microscopy. Additionally, this binding could be completed by the chemically synthesized APWHLSSQYSRT peptide, indicating that the binding capability was specific and conferred by the peptide sequence. Through the ligation of the peptide to CdSe-ZnS core-shell nanocrystals, we were able to, for the first time, target embryonic stem cells through peptide-conjugated quantum dots. CONCLUSIONS/SIGNIFICANCE: These data demonstrate that our established method of screening for embryonic stem cell specific binding peptides by phage display is feasible. Moreover, the peptide-conjugated quantum dots may be applicable for embryonic stem cell study and utilization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.