ArticlePloS one2010
Puma and Trail/Dr5 pathways control radiation-induced apoptosis in distinct populations of testicular progenitors.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- Blocking TRAIL-DR5 signaling pathway with soluble death receptor 5 fusion protein mitigates radiation-induced injury.Frontiers in pharmacology · 2023Article
- Spontaneous testicular atrophy occurs despite normal spermatogonial proliferation in a Tp53 knockout rat.Andrology · 2017Article
- TNFSF10/TRAIL regulates human T4 effector memory lymphocyte radiosensitivity and predicts radiation-induced acute and subacute dermatitis.Oncotarget · 2016Article
- Comprehensive identification of genes driven by ERV9-LTRs reveals TNFRSF10B as a re-activatable mediator of testicular cancer cell death.Cell death and differentiation · 2016Article
- The Trp53-Trp53inp1-Tnfrsf10b pathway regulates the radiation response of mouse spermatogonial stem cells.Stem cell reports · 2014Article
- Upregulation and nuclear translocation of testicular ghrelin protects differentiating spermatogonia from ionizing radiation injury.Cell death & disease · 2014Article
- The effects of antioxidants on gene expression following gamma-radiation (GR) and proton radiation (PR) in mice in vivo.Cell cycle (Georgetown, Tex.) · 2013Article
- Study on the apoptosis mechanism induced by T-2 toxin.PloS one · 2013Article
- Activation of Wnt/β-catenin signaling increases apoptosis in melanoma cells treated with trail.PloS one · 2013Article
- Slug inhibition upregulates radiation-induced PUMA activity leading to apoptosis in cholangiocarcinomas.Medical oncology (Northwood, London, England) · 2011Article
- The emerging role of matrix metalloproteases of the ADAM family in male germ cell apoptosis.Spermatogenesis · 2011Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spermatogonia- stem cells and progenitors of adult spermatogenesis- are killed through a p53-regulated apoptotic process after gamma-irradiation but the death effectors are still poorly characterized. Our data demonstrate that both intrinsic and extrinsic apoptotic pathways are involved, and especially that spermatogonia can be split into two main populations, according to apoptotic effectors. Following irradiation both Dr5 and Puma genes are upregulated in the alpha6-integrin-positive Side Population (SP) fraction, which is highly enriched in spermatogonia. Flow cytometric analysis confirms an increased number of Dr5-expressing SP cells, and Puma-beta isoform accumulates in alpha6-integrin positive cellular extracts, enriched in spermatogonia. Trail-/- or Puma-/- spermatogonia display a reduced sensitivity to radiation-induced apoptosis. The TUNEL kinetics strongly suggest that the extrinsic and intrinsic pathways, via Trail/Dr5 and Puma respectively, could be engaged in distinct subpopulations of spermatogonia. Indeed flow cytometric studies show that Dr5 receptor is constitutively present on more than half of the undifferentiated progenitors (Kit- alpha6+ SP) and half of the differentiated ones (Kit+ alpha6+ SP). In addition after irradiation, Puma is not detected in the Dr5-positive cellular fraction isolated by immunomagnetic purification, while Puma is present in the Dr5-negative cell extracts. In conclusion, adult testicular progenitors are divided into distinct sub-populations by apoptotic effectors, independently of progenitor types (immature Kit-negative versus mature Kit-positive), underscoring differential radiosensitivities characterizing the stem cell/progenitors compartment.
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