ArticleEukaryotic cell2010
Heterologous expression of Candida albicans cell wall-associated adhesins in Saccharomyces cerevisiae Reveals differential specificities in adherence and biofilm formation and in binding oral Streptococcus gordonii.
Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
74 citing papers in PubMed, 109 citations in OpenAlex.
- Bacterial-fungal interactions: connections and consequences.Essays in biochemistry · 2026Review
- Inhibiting biofilm growth on ammonium salt-functionalized or fluorinated voice prostheses silicone.Applied microbiology and biotechnology · 2026Article
- Constitutive ALS3 expression in Candida albicans has differential effects on yeast-locked tet-NRG1 and brg1Δ strains.Folia microbiologica · 2026Article
- Adhesin Als4112 promotes Candida auris skin colonization through interactions with keratinocytes and extracellular matrix proteins.Nature communications · 2025Article
- Review
- Is Oral Microflora Related to Development of Malfunction in Patients Using Voice Prosthesis?Journal of clinical medicine · 2024Article
- Secreted Aspartic Proteinases: Key Factors inInternational journal of molecular sciences · 2024Review
- Glyceraldehyde 3-Phosphate Dehydrogenase on the Surface ofInternational journal of molecular sciences · 2024Article
- A review on antimicrobial strategies in mitigating biofilm-associated infections on medical implants.Current research in microbial sciences · 2024Review
- Promising immunotherapeutic targets for treating candidiasis.Frontiers in cellular and infection microbiology · 2024Review
- The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans.NPJ biofilms and microbiomes · 2023Article
- Heterologous Expression of Candida Antifungal Target Genes in the Model Organism Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Interkingdom assemblages in human saliva display group-level surface mobility and disease-promoting emergent functions.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Interactions betweenFrontiers in microbiology · 2022Review
- Review
- Proteinous Components of Neutrophil Extracellular Traps Are Arrested by the Cell Wall Proteins ofCells · 2021Article
- Article
- The Role ofFrontiers in cellular and infection microbiology · 2021Review
- Cross-Kingdom Cell-to-Cell Interactions in Cariogenic Biofilm Initiation.Journal of dental research · 2021Article
- Evolutionary Overview of Molecular Interactions and Enzymatic Activities in the Yeast Cell Walls.International journal of molecular sciences · 2020Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
Abstract
Colonization and infection of the human host by opportunistic pathogen Candida albicans derive from an ability of this fungus to colonize mucosal tissues and prosthetic devices within the polymicrobial communities present. To determine the functions of C. albicans cell wall proteins in interactions with host or bacterial molecules, Saccharomyces cerevisiae was utilized as a surrogate host to express C. albicans cell wall proteins Als3p, Eap1p, Hwp1p, and Rbt1p. Salivary pellicle and fibrinogen were identified as novel substrata for Als3p and Hwp1p, while only Als3p mediated adherence of S. cerevisiae to basement membrane collagen type IV. Parental S. cerevisiae cells failed to form biofilms on salivary pellicle, polystyrene, or silicone, but cells expressing Als3p or Hwp1p exhibited significant attachment to each surface. Virulence factor Rbt1p also conferred lower-level binding to salivary pellicle and polystyrene. S. cerevisiae cells expressing Eap1p formed robust biofilms upon polystyrene surfaces but not salivary pellicle. Proteins Als3p and Eap1p, and to a lesser degree Hwp1p, conferred upon S. cerevisiae the ability to bind cells of the oral primary colonizing bacterium Streptococcus gordonii. These interactions, which occurred independently of amyloid aggregate formation, provide the first examples of specific C. albicans surface proteins serving as receptors for bacterial adhesins. Streptococcus gordonii did not bind parental S. cerevisiae or cells expressing Rbt1p. Taken collectively, these data suggest that a network of cell wall proteins comprising Als3p, Hwp1p, and Eap1p, with complementary adhesive functions, promotes interactions of C. albicans with host and bacterial molecules, thus leading to effective colonization within polymicrobial communities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.