ArticleThe Journal of biological chemistry2010
Cysteine is not the sulfur source for iron-sulfur cluster and methionine biosynthesis in the methanogenic archaeon Methanococcus maripaludis.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 115 citations in OpenAlex.
- Article
- Phage hijacks host phosphorothioate DNA modification machinery to circumvent bacterial Ssp defences.Nature microbiology · 2026Article
- The HypA and HypB metallochaperones from Methanococcus maripaludis have unique metal-binding properties and a distinct nickel transfer mechanism.The Journal of biological chemistry · 2026Article
- Intricacies in iron-sulfur cluster function and biogenesis: functional versatility, sulfur sources, and enzyme specificity.RSC chemical biology · 2026Review
- Metabolic adaptation of a methanogen to a ferruginous environment associated with intracellular Fe- and S-rich nanoparticles.Frontiers in microbiology · 2026Article
- Hydrogen sulfide acts as a sulfur source for iron sulfur cluster biosynthesis in cysteine desulfurase-deficientFrontiers in microbiology · 2026Article
- Evolution, structure and function of L-cysteine desulfidase, an enzyme involved in sulfur metabolism in the methanogenic archeon Methanococcus maripaludis.Communications biology · 2025Article
- A minimal SufBMicrobiology spectrum · 2025Article
- SmsB-SmsC machinery inApplied and environmental microbiology · 2025Article
- Archaeal protein containing domain of unknown function 2193 undergoes oligomeric reconfiguration upon iron-sulfur cluster binding.FEBS letters · 2025Article
- Ancestral [Fe-S] biogenesis system SMS has a unique mechanism of cluster assembly and sulfur utilization.PLoS biology · 2025Article
- Anaerobic breviate protist survival in microcosms depends on microbiome metabolic function.The ISME journal · 2025Article
- Alternative sources of molybdenum for Methanococcus maripaludis and their implication for the evolution of molybdoenzymes.Communications biology · 2024Article
- Proteomic strategies to interrogate the Fe-S proteome.Biochimica et biophysica acta. Molecular cell research · 2024Review
- Article
- Impact of mineral and non-mineral sources of iron and sulfur on the metalloproteome ofApplied and environmental microbiology · 2024Article
- Chemical Antiquity in Metabolism.Accounts of chemical research · 2024Article
- Article
- Extreme mitochondrial reduction in a novel group of free-living metamonads.Nature communications · 2024Article
- A shift between mineral and nonmineral sources of iron and sulfur causes proteome-wide changes inMicrobiology spectrum · 2024Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three multiprotein systems are known for iron-sulfur (Fe-S) cluster biogenesis in prokaryotes and eukaryotes as follows: the NIF (nitrogen fixation), the ISC (iron-sulfur cluster), and the SUF (mobilization of sulfur) systems. In all three, cysteine is the physiological sulfur source, and the sulfur is transferred from cysteine desulfurase through a persulfidic intermediate to a scaffold protein. However, the biochemical nature of the sulfur source for Fe-S cluster assembly in archaea is unknown, and many archaea lack homologs of cysteine desulfurases. Methanococcus maripaludis is a methanogenic archaeon that contains a high amount of protein-bound Fe-S clusters (45 nmol/mg protein). Cysteine in this archaeon is synthesized primarily via the tRNA-dependent SepRS/SepCysS pathway. When a ΔsepS mutant (a cysteine auxotroph) was grown with (34)S-labeled sulfide and unlabeled cysteine, <8% of the cysteine, >92% of the methionine, and >87% of the sulfur in the Fe-S clusters in proteins were labeled, suggesting that the sulfur in methionine and Fe-S clusters was derived predominantly from exogenous sulfide instead of cysteine. Therefore, this investigation challenges the concept that cysteine is always the sulfur source for Fe-S cluster biosynthesis in vivo and suggests that Fe-S clusters are derived from sulfide in those organisms, which live in sulfide-rich habitats.
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