ArticleMicrovascular research2010
Calpain- and talin-dependent control of microvascular pericyte contractility and cellular stiffness.
Article in Microvascular research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- Single-pericyte nanomechanics measured by contraction cytometry.APL bioengineering · 2024Article
- Review
- Review
- Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease.FEBS open bio · 2021Article
- Reactive species-induced microvascular dysfunction in ischemia/reperfusion.Free radical biology & medicine · 2019Review
- Co-exposure subacute toxicity of silica nanoparticles and lead acetate on cardiovascular system.International journal of nanomedicine · 2018Article
- Attenuation of Blood-Brain Barrier Breakdown and Hyperpermeability by Calpain Inhibition.The Journal of biological chemistry · 2016Article
- Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting.Physical biology · 2016Article
- Calpain inhibition improves collateral-dependent perfusion in a hypercholesterolemic swine model of chronic myocardial ischemia.The Journal of thoracic and cardiovascular surgery · 2016Article
- Talin determines the nanoscale architecture of focal adhesions.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Pericyte chemomechanics and the angiogenic switch: insights into the pathogenesis of proliferative diabetic retinopathy?Investigative ophthalmology & visual science · 2015Article
- Propranolol targets the contractility of infantile haemangioma-derived pericytes.The British journal of dermatology · 2014Article
- Pericyte contractility controls endothelial cell cycle progression and sprouting: insights into angiogenic switch mechanics.American journal of physiology. Cell physiology · 2014Article
- Cooperation between human fibrocytes and endothelial colony-forming cells increases angiogenesis via the CXCR4 pathway.Thrombosis and haemostasis · 2014Article
- Silica nanoparticles enhance autophagic activity, disturb endothelial cell homeostasis and impair angiogenesis.Particle and fibre toxicology · 2014Article
- Regulation of blood flow in the retinal trilaminar vascular network.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Pericyte-endothelial crosstalk: implications and opportunities for advanced cellular therapies.Translational research : the journal of laboratory and clinical medicine · 2014Review
- Pericytes derived from adipose-derived stem cells protect against retinal vasculopathy.PloS one · 2013Article
- Calpain inhibition preserves talin and attenuates right heart failure in acute pulmonary hypertension.American journal of respiratory cell and molecular biology · 2012Article
- Vascular complications and diabetes: current therapies and future challenges.Journal of ophthalmology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Pericytes surround capillary endothelial cells and exert contractile forces modulating microvascular tone and endothelial growth. We previously described pericyte contractile phenotype to be Rho GTPase- and α-smooth muscle actin (αSMA)-dependent. However, mechanisms mediating adhesion-dependent shape changes and contractile force transduction remain largely equivocal. We now report that the neutral cysteine protease, calpain, modulates pericyte contractility and cellular stiffness via talin, an integrin-binding and F-actin associating protein. Digital imaging and quantitative analyses of living cells reveal significant perturbations in contractile force transduction detected via deformation of silicone substrata, as well as perturbations of mechanical stiffness in cellular contractile subdomains quantified via atomic force microscope (AFM)-enabled nanoindentation. Pericytes overexpressing GFP-tagged talin show significantly enhanced contractility (~two-fold), which is mitigated when either the calpain-cleavage resistant mutant talin L432G or vinculin are expressed. Moreover, the cell-penetrating, calpain-specific inhibitor termed CALPASTAT reverses talin-enhanced, but not Rho GTP-dependent, contractility. Interestingly, our analysis revealed that CALPASTAT, but not its inactive mutant, alters contractile cell-driven substrata deformations while increasing mechanical stiffness of subcellular contractile regions of these pericytes. Altogether, our results reveal that calpain-dependent cleavage of talin modulates cell contractile dynamics, which in pericytes may prove instrumental in controlling normal capillary function or microvascular pathophysiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.