ArticleAnnals of clinical microbiology and antimicrobials2010
Identification of Staphylococcus aureus: DNase and Mannitol salt agar improve the efficiency of the tube coagulase test.
Article in Annals of clinical microbiology and antimicrobials, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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103 citing papers in PubMed.
- Virulence determinants and multidrug resistance profiles of biofilm-forming Staphylococcus aureus from healthy orthopedic surgical personnel.Antonie van Leeuwenhoek · 2026Article
- Genomic insights into specialized and primary metabolism gene clusters in methicillin-resistant Staphylococcus aureus from Egyptian community settings.BMC genomics · 2026Article
- Prevalence and risk factors of antimicrobial resistance inOne health (Amsterdam, Netherlands) · 2026Article
- Virulence Profiling of Multidrug-Resistant ClinicalMicrobial drug resistance (Larchmont, N.Y.) · 2026Article
- Pathoadaptive evolution and clonal dissemination of community- associated methicillin-resistant Staphylococcus aureus in Egypt.BMC infectious diseases · 2026Article
- Antibiotic resistance patterns of methicillin-susceptible Staphylococcus aureus and methicillin-resistant Staphylococcus aureus isolates in keratitis: Insights from an 11-year analysis.Indian journal of ophthalmology · 2026Article
- Molecular diagnosis of sepsis in hospital patients: assessment of the Agata SepsisBMC infectious diseases · 2026Article
- Preparation of silibinin- dendrimer-stabilized Au nanoparticles for decreasing vancomycin resistance in S. aureus clinical isolates.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Enterotoxin and biofilm producing Staphylococcus aureus and MRSA isolates from animal source foods sold in Assam.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- First Report ofMicroorganisms · 2026Article
- Molecular Characterization of Staphylococcus aureus and Coagulase-Negative Staphylococci Isolates at a Rural Hospital in Southern Malawi.BioMed research international · 2026Article
- Effectiveness of cold atmospheric plasma on Staphylococcus Aureus colonies on living animal tissue surface.PloS one · 2026Article
- Risk Factors Associated With Methicillin-ResistantThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2026Article
- Synergistic Anti-Biofilm and Bactericidal Activity of Ethanol and Chlorhexidine Combined with EDTA AgainstInfection and drug resistance · 2026Article
- Antibacterial and antibiofilm activity of platelet-rich plasma under different activation conditions against multidrug-resistant MRSA isolated from human skin abscesses.BMC biotechnology · 2025Article
- Evaluation of Antimicrobial and Antibiofilm Activity ofMicroorganisms · 2025Article
- Intersecting realms of resistance: the epidemiology of Staphylococcus aureus carriage and multidrug resistance in primary healthcare settings in Recife, Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Prevalence of methicillin-resistantIJID regions · 2025Article
- Enhancing the Antimicrobial Effectiveness of Zinc Oxide Nanoparticles Against Staphylococcus aureus, Through Combination with Potassium and Zingerone.Current microbiology · 2025Article
- Antimicrobial susceptibility pattern of Staphylococcus Aureus isolated from clinical specimens at University of Gondar comprehensive specialized referral hospital northwest Ethiopia; a retrospective study.BMC research notes · 2025Article
43 more citing papers are in PubMed but not listed here.
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Funding
Abstract
backgroundThe ideal identification of Staphylococcus aureus clinical isolates requires a battery of tests and this is costly in resource limited settings. In many developing countries, the tube coagulase test is usually confirmatory for S. aureus and is routinely done using either human or sheep plasma. This study evaluated Mannitol salt agar and the deoxyribonuclease (DNase) test for improving the efficiency of the tube coagulase test in resource limited settings. The efficiency of human and sheep plasma with tube coagulase tests was also evaluated.
methodsOne hundred and eighty Gram positive, Catalase positive cocci occurring in pairs, short chains or clusters were subjected to growth on Mannitol salt agar, deoxyribonuclease and tube coagulase tests. Of these, isolates that were positive for at least two of the three tests (n = 60) were used to evaluate the performance of the tube coagulase test for identification of S. aureus, using PCR-amplification of the nuc gene as a gold standard.
resultsHuman plasma was more sensitive than sheep plasma for the tube coagulase test (sensitivity of 91% vs. 81% respectively), but both plasmas had very low specificity (11% and 7% respectively). The sensitivity and specificity of the tube coagulase test (human plasma) was markedly improved when Mannitol salt agar and DNase were introduced as a tri-combination test for routine identification of Staphylococcus aureus (100% specificity and 75% sensitivity). The specificity and sensitivity of Mannitol salt agar/DNase/tube coagulase (sheep plasma) combination was 100% and 67%, respectively.
conclusionThe efficiency of the tube coagulase test can be markedly improved by sequel testing of the isolates with Mannitol salt agar, DNase and Tube coagulase. There is no single phenotypic test (including tube coagulase) that can guarantee reliable results in the identification of Staphylococcus aureus.
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