ArticleInternational journal of microbiology2010
Frequency of drug resistance gene amplification in clinical leishmania strains.
Article in International journal of microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
- Effectiveness of the sesquiterpene (-)-α-bisabolol in dogs with naturally acquired canine leishmaniosis: an exploratory clinical trial.Veterinary research communications · 2018Trial
- Molecular evolution in light of regulatory-coding epistasis.EMBO reports · 2026Review
- Using Q-methodology to understand the perspectives and practical experiences of dermatologists about treatment difficulties of cutaneous leishmaniasis.BMC infectious diseases · 2020Article
- Genome wide comparison of Ethiopian Leishmania donovani strains reveals differences potentially related to parasite survival.PLoS genetics · 2018Article
- Treatment Outcome of the Drug-resistant Zoonotic Cutaneous Leishmaniasis by Glucantime.Advanced biomedical research · 2017Article
- Geographical Distribution of MDR1 Expression in Leishmania Isolates, from Greece and Cyprus, Measured by the Rhodamine-123 Efflux Potential of the Isolates, Using Flow Cytometry.The American journal of tropical medicine and hygiene · 2016Article
- P-glycoprotein A Gene Expression in Glucantime-Resistant and Sensitive Leishmania major (MRHO/IR/75/ER).Iranian journal of parasitology · 2014Article
- Heterogeneity of molecular resistance patterns in antimony-resistant field isolates of Leishmania species from the western Mediterranean area.Antimicrobial agents and chemotherapy · 2014Article
- Frequency of MDR1-related p-gp overexpression in Greek Leishmania isolates.Parasitology research · 2014Article
- Role of efflux pumps and intracellular thiols in natural antimony resistant isolates of Leishmania donovani.PloS one · 2013Article
- Application of the NucliSENS easyMAG system for nucleic acid extraction: optimization of DNA extraction for molecular diagnosis of parasitic and fungal diseases.Parasite (Paris, France) · 2013Article
- Drug resistance in natural isolates of Leishmania donovani s.l. promastigotes is dependent of Pgp170 expression.PloS one · 2013Article
- Gene duplication as a mechanism of genomic adaptation to a changing environment.Proceedings. Biological sciences · 2012Review
- Antimony resistance in leishmania, focusing on experimental research.Journal of tropical medicine · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Experimental studies about Leishmania resistance to metal and antifolates have pointed out that gene amplification is one of the main mechanisms of drug detoxification. Amplified genes code for adenosine triphosphate-dependent transporters (multidrug resistance and P-glycoproteins P), enzymes involved in trypanothione pathway, particularly gamma glutamyl cysteine synthase, and others involved in folates metabolism, such as dihydrofolate reductase and pterine reductase. The aim of this study was to detect and quantify the amplification of these genes in clinical strains of visceral leishmaniasis agents: Leishmania infantum, L. donovani, and L. archibaldi. Relative quantification experiments by means of real-time polymerase chain reaction showed that multidrug resistance gene amplification is the more frequent event. For P-glycoproteins P and dihydrofolate reductase genes, level of amplification was comparable to the level observed after in vitro selection of resistant clones. Gene amplification is therefore a common phenomenon in wild strains concurring to Leishmania genomic plasticity. This finding, which corroborates results of experimental studies, supports a better understanding of metal resistance selection and spreading in endemic areas.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.