ArticlePLoS neglected tropical diseases2010
FMRFamide-like peptides (FLPs) enhance voltage-gated calcium currents to elicit muscle contraction in the human parasite Schistosoma mansoni.
Article in PLoS neglected tropical diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Review
- Discovery of Schistosoma mekongi circulating proteins and antigens in infected mouse sera.PloS one · 2022Article
- Identification of Low Molecular Weight Proteins and Peptides fromBiomolecules · 2021Article
- Anti-schistosomal action of the calcium channel agonist FPL-64176.International journal for parasitology. Drugs and drug resistance · 2019Article
- The in vivo transcriptome of Schistosoma mansoni in the prominent vector species Biomphalaria pfeifferi with supporting observations from Biomphalaria glabrata.PLoS neglected tropical diseases · 2019Article
- Chemotactic migration of newly excysted juvenile Clonorchis sinensis is suppressed by neuro-antagonists.PLoS neglected tropical diseases · 2019Article
- Utilizing the planarian voltage-gated ion channel transcriptome to resolve a role for a CaBiochimica et biophysica acta. Molecular cell research · 2017Article
- Protein kinase C and extracellular signal-regulated kinase regulate movement, attachment, pairing and egg release in Schistosoma mansoni.PLoS neglected tropical diseases · 2014Article
- 'Death and axes': unexpected Ca²⁺ entry phenologs predict new anti-schistosomal agents.PLoS pathogens · 2014Article
- Ca²⁺ channels and praziquantel: a view from the free world.Parasitology international · 2013Review
- Review
- Parasite neuropeptide biology: Seeding rational drug target selection?International journal for parasitology. Drugs and drug resistance · 2012Review
- Opposing roles of voltage-gated Ca2+ channels in neuronal control of regenerative patterning.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- The N terminus of a schistosome beta subunit regulates inactivation and current density of a Cav2 channel.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Schistosomes are amongst the most important and neglected pathogens in the world, and schistosomiasis control relies almost exclusively on a single drug. The neuromuscular system of schistosomes is fertile ground for therapeutic intervention, yet the details of physiological events involved in neuromuscular function remain largely unknown. Short amidated neuropeptides, FMRFamide-like peptides (FLPs), are distributed abundantly throughout the nervous system of every flatworm examined and they produce potent myoexcitation. Our goal here was to determine the mechanism by which FLPs elicit contractions of schistosome muscle fibers. Contraction studies showed that the FLP Tyr-Ile-Arg-Phe-amide (YIRFamide) contracts the muscle fibers through a mechanism that requires Ca(2+) influx through sarcolemmal voltage operated Ca(2+) channels (VOCCs), as the contractions are inhibited by classical VOCC blockers nicardipine, verapamil and methoxyverapamil. Whole-cell patch-clamp experiments revealed that inward currents through VOCCs are significantly and reversibly enhanced by the application of 1 microM YIRFamide; the sustained inward currents were increased to 190% of controls and the peak currents were increased to 180%. In order to examine the biochemical link between the FLP receptor and the VOCCs, PKC inhibitors calphostin C, RO 31-8220 and chelerythrine were tested and all produced concentration dependent block of the contractions elicited by 1 microM YIRFamide. Taken together, the data show that FLPs elicit contractions by enhancing Ca(2+) influx through VOCC currents using a PKC-dependent pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.