ArticlePLoS neglected tropical diseases2010
The Echinococcus granulosus antigen B gene family comprises at least 10 unique genes in five subclasses which are differentially expressed.
Article in PLoS neglected tropical diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 89 citations in OpenAlex.
- Article
- Validation of reference genes for RT-qPCR relative expression analysis during cyst-to-early adult development of Taenia solium.PLoS neglected tropical diseases · 2026Article
- Echinococcus granulosus antigen B regulates T-cell function through inhibition of signal transducer and activator of transcription 3 in experimental immune thrombocytopenia.British journal of haematology · 2025Article
- Therapeutic effect of recombinant Echinococcus granulosus antigen B subunit 2 protein on sepsis in a mouse model.Parasites & vectors · 2024Article
- Modulatory actions ofFrontiers in cellular and infection microbiology · 2024Article
- Dimerization, host-parasite communication and expression studies of an Echinococcus granulosus 2DBD nuclear receptor.Parasitology research · 2023Article
- Antigen B modulates anti-inflammatory cytokines in the EAE model of multiple sclerosis.Brain and behavior · 2023Article
- Function of lipid binding proteins of parasitic helminths: still a long road.Parasitology research · 2022Review
- Article
- Evaluation of the sensitivity and specificity of GST-tagged recombinant antigens 2B2t, Ag5t and DIPOL in ELISA for the diagnosis and follow up of patients with cystic echinococcosis.PLoS neglected tropical diseases · 2020Article
- Survey of echinococcoses in southeastern Qinghai Province, China, and serodiagnostic insights of recombinant Echinococcus granulosus antigen B isoforms.Parasites & vectors · 2019Article
- Antigen B from Echinococcus granulosus enters mammalian cells by endocytic pathways.PLoS neglected tropical diseases · 2018Article
- Genetic Variability of Antigen B8/1 amongReports of biochemistry & molecular biology · 2018Article
- Comparison ofOncotarget · 2017Article
- Liver cystic echinococcosis and human host immune and autoimmune follow-up: A review.World journal of hepatology · 2017Review
- Characterisation of Antigen B Protein Species Present in the Hydatid Cyst Fluid of Echinococcus canadensis G7 Genotype.PLoS neglected tropical diseases · 2017Article
- Cloning and characterization of an Echinococcus granulosus ecdysteroid hormone nuclear receptor HR3-like gene.Parasite (Paris, France) · 2017Article
- Echinococcus granulosus Antigen B binds to monocytes and macrophages modulating cell response to inflammation.Parasites & vectors · 2016Article
- An Echinococcus multilocularis Antigen B3 Proteoform That Shows Specific Antibody Responses to Active-Stage Alveolar Echinococcosis.Journal of clinical microbiology · 2015Article
- Lipid-free antigen B subunits from echinococcus granulosus: oligomerization, ligand binding, and membrane interaction properties.PLoS neglected tropical diseases · 2015Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAntigen B (EgAgB) is a major protein produced by the metacestode cyst of Echinococcus granulosus, the causative agent of cystic hydatid disease. This protein has been shown to play an important role in modulating host immune responses, although its precise biological function still remains unknown. It is generally accepted that EgAgB is comprised of a gene family of five subfamilies which are highly polymorphic, but the actual number of genes present is unknown. METHODOLOGY/PRINCIPAL
findingsBased on published sequences for the family, we designed specific primers for each subfamily and used PCR to amplify them from genomic DNA isolated from individual mature adult worms (MAW) taken from an experimentally infected dog in China and individual larval protoscoleces (PSC) excised from a single hydatid cyst taken from an Australian kangaroo. We then used real-time PCR to measure expression of each of the genes comprising the five EgAgB subfamilies in all life-cycle stages including the oncosphere (ONC). CONCLUSIONS/SIGNIFICANCE: Based on sequence alignment analysis, we found that the EgAgB gene family comprises at least ten unique genes. Each of the genes was identical in both larval and adult E. granulosus isolates collected from two geographical areas (different continents). DNA alignment comparisons with EgAgB sequences deposited in GenBank databases showed that each gene in the gene family is highly conserved within E. granulosus, which contradicts previous studies claiming significant variation and polymorphism in EgAgB. Quantitative PCR analysis revealed that the genes were differentially expressed in different life-cycle stages of E. granulosus with EgAgB3 expressed predominantly in all stages. These findings are fundamental for determining the expression and the biological function of antigen B.
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