ArticleProceedings of the National Academy of Sciences of the United States of America2010
Fast live simultaneous multiwavelength four-dimensional optical microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
89 citing papers in PubMed, 195 citations in OpenAlex.
- Mitotic kinase regulation of DNA replication forks.bioRxiv : the preprint server for biology · 2026Article
- Exploring Biophysics at the Membrane with Single-Molecule TIRF Microscopy.Advances in experimental medicine and biology · 2026Review
- High-intensity light disrupts intracellular organelle dynamics via microtubule depolymerization.Scientific reports · 2025Article
- Single-shot quantitative phase microscopy: a multi-functional tool for cell analysis.Biomedical optics express · 2024Article
- In vivo organoid growth monitoring by stimulated Raman histology.Npj imaging · 2024Article
- Observing ER Dynamics over Long Timescales Using Light Sheet Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Optogenetic control of YAP reveals a dynamic communication code for stem cell fate and proliferation.Nature communications · 2023Article
- Optimal sparsity allows reliable system-aware restoration of fluorescence microscopy images.Science advances · 2023Article
- Dynamics of nuclear architecture during early embryonic development and lessons from liveimaging.Developmental cell · 2023Review
- SPITFIR(e): a supermaneuverable algorithm for fast denoising and deconvolution of 3D fluorescence microscopy images and videos.Scientific reports · 2023Article
- Kinetic analysis of synaptonemal complex dynamics during meiosis of yeastFrontiers in cell and developmental biology · 2023Article
- Visualizing MyoD Oscillations in Muscle Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- DNA damage reduces heterogeneity and coherence of chromatin motions.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Bringing to light the physiological and pathological firing patterns of human induced pluripotent stem cell-derived neurons using optical recordings.Frontiers in cellular neuroscience · 2022Article
- Optimizing Long-Term Live Cell Imaging.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Phototoxicity induced in living HeLa cells by focused femtosecond laser pulses: a data-driven approach.Biomedical optics express · 2021Article
- An adaptive microscope for the imaging of biological surfaces.Light, science & applications · 2021Article
- Python-Microscope - a new open-source Python library for the control of microscopes.Journal of cell science · 2021Article
- Heterodyne Brillouin microscopy for biomechanical imaging.Biomedical optics express · 2021Article
- Performance of deep learning restoration methods for the extraction of particle dynamics in noisy microscopy image sequences.Molecular biology of the cell · 2021Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Commented on by
Authors and funding
18 authors at 9 institutions in 4 countries.
Funding
Abstract
Live fluorescence microscopy has the unique capability to probe dynamic processes, linking molecular components and their localization with function. A key goal of microscopy is to increase spatial and temporal resolution while simultaneously permitting identification of multiple specific components. We demonstrate a new microscope platform, OMX, that enables subsecond, multicolor four-dimensional data acquisition and also provides access to subdiffraction structured illumination imaging. Using this platform to image chromosome movement during a complete yeast cell cycle at one 3D image stack per second reveals an unexpected degree of photosensitivity of fluorophore-containing cells. To avoid perturbation of cell division, excitation levels had to be attenuated between 100 and 10,000× below the level normally used for imaging. We show that an image denoising algorithm that exploits redundancy in the image sequence over space and time allows recovery of biological information from the low light level noisy images while maintaining full cell viability with no fading.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.