ArticleNucleic acids research2010
A flexible loop in yeast ribosomal protein L11 coordinates P-site tRNA binding.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 33 citations in OpenAlex.
- The role of ribosomal protein networks in ribosome dynamics.Nucleic acids research · 2025Article
- Review
- A core network in the SARS-CoV-2 nucleocapsid NTD mediates structural integrity and selective RNA-binding.Nature communications · 2024Article
- Protein Fold Usages in Ribosomes: Another Glance to the Past.International journal of molecular sciences · 2024Article
- The P-Site Loop of the Universally Conserved Bacterial Ribosomal Protein L5 Is Required for Maintaining Both Translation Rate and Fidelity.International journal of molecular sciences · 2023Article
- A small ribosome-associated ncRNA globally inhibits translation by restricting ribosome dynamics.RNA biology · 2021Article
- Evolution of ribosomal protein network architectures.Scientific reports · 2021Article
- Structural basis for ribosome recycling by RRF and tRNA.Nature structural & molecular biology · 2020Article
- Trypanosoma brucei L11 Is Essential to Ribosome Biogenesis and Interacts with the Kinetoplastid-Specific Proteins P34 and P37.mSphere · 2019Article
- Ribosomal protein eL24, involved in two intersubunit bridges, stimulates translation initiation and elongation.Nucleic acids research · 2019Article
- Multi-protein bridging factor 1(Mbf1), Rps3 and Asc1 prevent stalled ribosomes from frameshifting.eLife · 2018Article
- Translation Elongation and Recoding in Eukaryotes.Cold Spring Harbor perspectives in biology · 2018Review
- Tracking fluctuation hotspots on the yeast ribosome through the elongation cycle.Nucleic acids research · 2017Article
- The eukaryote-specific N-terminal extension of ribosomal protein S31 contributes to the assembly and function of 40S ribosomal subunits.Nucleic acids research · 2016Article
- The Functional Role of eL19 and eB12 Intersubunit Bridge in the Eukaryotic Ribosome.Journal of molecular biology · 2016Article
- Article
- Structure of the human 80S ribosome.Nature · 2015Article
- Ribosomes in the balance: structural equilibrium ensures translational fidelity and proper gene expression.Nucleic acids research · 2014Article
- Structure of the mammalian ribosomal pre-termination complex associated with eRF1.eRF3.GDPNP.Nucleic acids research · 2014Article
- Eukaryotic rpL10 drives ribosomal rotation.Nucleic acids research · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
High-resolution structures reveal that yeast ribosomal protein L11 and its bacterial/archael homologs called L5 contain a highly conserved, basically charged internal loop that interacts with the peptidyl-transfer RNA (tRNA) T-loop. We call this the L11 'P-site loop'. Chemical protection of wild-type ribosome shows that that the P-site loop is inherently flexible, i.e. it is extended into the ribosomal P-site when this is unoccupied by tRNA, while it is retracted into the terminal loop of 25S rRNA Helix 84 when the P-site is occupied. To further analyze the function of this structure, a series of mutants within the P-site loop were created and analyzed. A mutant that favors interaction of the P-site loop with the terminal loop of Helix 84 promoted increased affinity for peptidyl-tRNA, while another that favors its extension into the ribosomal P-site had the opposite effect. The two mutants also had opposing effects on binding of aa-tRNA to the ribosomal A-site, and downstream functional effects were observed on translational fidelity, drug resistance/hypersensitivity, virus maintenance and overall cell growth. These analyses suggest that the L11 P-site loop normally helps to optimize ribosome function by monitoring the occupancy status of the ribosomal P-site.
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