ArticleAnalytical chemistry2010
Improved sensitivity of DNA microarrays using photonic crystal enhanced fluorescence.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Portable, smartphone-linked, and miniaturized photonic resonator absorption microscope (PRAM Mini) for point-of-care diagnostics.Biomedical optics express · 2024Article
- Microscopies Enabled by Photonic Metamaterials.Sensors (Basel, Switzerland) · 2022Review
- An Automated Microfluidic Assay for Photonic Crystal Enhanced Detection and Analysis of an Antiviral Antibody Cancer Biomarker in Serum.IEEE sensors journal · 2018Article
- Comparison of Methods Study between a Photonic Crystal Biosensor and Certified ELISA to Measure Biomarkers of Iron Deficiency in Chronic Kidney Disease Patients.Sensors (Basel, Switzerland) · 2017Article
- Recent Advances in Biosensing With Photonic Crystal Surfaces: A Review.IEEE sensors journal · 2016Article
- Application of photonic crystal enhanced fluorescence to detection of low serum concentrations of human IgE antibodies specific for a purified cat allergen (Fel D1).Biosensors & bioelectronics · 2016Article
- Direct detection of transcription factors in cotyledons during seedling development using sensitive silicon-substrate photonic crystal protein arrays.Plant physiology · 2015Article
- Two-dimensional photonic crystals for sensitive microscale chemical and biochemical sensing.Lab on a chip · 2015Review
- Article
- Photonic crystal enhancement of a homogeneous fluorescent assay using submicron fluid channels fabricated by E-jet patterning.Journal of biophotonics · 2014Article
- Application of microarray technology for the detection of intracranial bacterial infection.Experimental and therapeutic medicine · 2014Article
- Nanostructured surfaces and detection instrumentation for photonic crystal enhanced fluorescence.Sensors (Basel, Switzerland) · 2013Review
- Multiplexed cancer biomarker detection using quartz-based photonic crystal surfaces.Analytical chemistry · 2012Article
- Spatially selective photonic crystal enhanced fluorescence and application to background reduction for biomolecule detection assays.Optics express · 2011Article
- Application of photonic crystal enhanced fluorescence to cancer biomarker microarrays.Analytical chemistry · 2011Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
DNA microarrays are used to profile changes in gene expression between samples in a high-throughput manner, but measurements of genes with low expression levels can be problematic with standard microarray substrates. In this work, we expand the detection capabilities of a standard microarray experiment using a photonic crystal (PC) surface that enhances fluorescence observed from microarray spots. This PC is inexpensively and uniformly fabricated using a nanoreplica molding technique, with very little variation in its optical properties within- and between-devices. By using standard protocols to process glass microarray substrates in parallel with PCs, we evaluated the impact of this substrate on a one-color microarray experiment comparing gene expression in two developmental stages of Glycine max. The PCs enhanced the signal-to-noise ratio observed from microarray spots by 1 order of magnitude, significantly increasing the number of genes detected above substrate fluorescence noise. PC substrates more than double the number of genes classified as differentially expressed, detecting changes in expression even for low expression genes. This approach increases the dynamic range of a surface-bound fluorescence-based assay to reliably quantify small quantities of DNA that would be impossible with standard substrates.
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Registered trials
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