ArticleProtoplasma2011
TMBP200, a XMAP215 homologue of tobacco BY-2 cells, has an essential role in plant mitosis.
Article in Protoplasma, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed.
- Preprophase-band positioning in isolated tobacco BY-2 cells: evidence for a principal role of nucleus-cell cortex interaction in default division-plane selection.Protoplasma · 2019Article
- Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- TOG-tubulin binding specificity promotes microtubule dynamics and mitotic spindle formation.The Journal of cell biology · 2017Article
- The functions of the cytoskeleton and associated proteins during mitosis and cytokinesis in plant cells.Frontiers in plant science · 2015Review
- The same, but different--a bird's-eye view on mitosis.Protoplasma · 2011Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
TMBP200 from tobacco BY-2 cells is a member of the highly conserved family of microtubule-associated proteins that includes Xenopus XMAP215, human TOGp, and Arabidopsis MOR1/GEM1. XMAP215 homologues have an essential role in spindle assembly and function in animals and yeast, but their role in plant mitosis is not fully clarified. Here, we show by immunoblot analysis that TMBP200 levels in synchronously cultured BY-2 cells increased when the cells entered mitosis, thus indicating that TMBP200 plays an important role in mitosis in tobacco. To investigate the role of TMBP200 in mitosis, we employed inducible RNA interference to silence TMBP200 expression in BY-2 cells. The resulting depletion of TMBP200 caused severe defects in bipolar spindle formation and resulted in the appearance of multinucleated cells with variable-sized nuclei. This finding indicates that TMBP200 has an essential role in bipolar spindle formation and function.
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Registered trials
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