ArticleArteriosclerosis, thrombosis, and vascular biology2010
Endothelium-dependent coronary vasodilatation requires NADPH oxidase-derived reactive oxygen species.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 65 citations in OpenAlex.
- Aging is associated with altered estrogen receptor expression and alters redox protein balance in human female skeletal muscle.GeroScience · 2026Article
- Female patients exhibit altered vasopressin-induced coronary microvascular contractile response and molecular signaling following cardiac surgery.American journal of surgery · 2025Article
- Endothelial Autophagy Dysregulation in Diabetes.Cells · 2023Review
- Coronary Microvascular Dysfunction in Diabetes Mellitus: Pathogenetic Mechanisms and Potential Therapeutic Options.Biomedicines · 2022Review
- Inhibition of Endoplasmic Reticulum Stress Improves Acetylcholine-Mediated Relaxation in the Aorta of Type-2 Diabetic Rats.Molecules (Basel, Switzerland) · 2022Article
- Mechanisms and clinical implications of endothelium-dependent vasomotor dysfunction in coronary microvasculature.American journal of physiology. Heart and circulatory physiology · 2022Review
- Optimization of mito-roGFP protocol to measure mitochondrial oxidative status in human coronary artery endothelial cells.STAR protocols · 2021Article
- Delivery of a mitochondria-targeted antioxidant from biocompatible, polymeric nanofibrous scaffolds.FEBS open bio · 2021Article
- Coronary endothelial dysfunction prevented by small-conductance calcium-activated potassium channel activator in mice and patients with diabetes.The Journal of thoracic and cardiovascular surgery · 2020Article
- The Relationship Between Reactive Oxygen Species and Endothelial Cell Metabolism.Frontiers in chemistry · 2020Review
- Subcellular Reactive Oxygen Species (ROS) in Cardiovascular Pathophysiology.Antioxidants (Basel, Switzerland) · 2018Review
- Genetic Alterations in Oxidant and Anti-Oxidant Enzymes in the Vascular System.Frontiers in cardiovascular medicine · 2018Article
- Diabetes Upregulation of Cyclooxygenase 2 Contributes to Altered Coronary Reactivity After Cardiac Surgery.The Annals of thoracic surgery · 2017Article
- Mitochondrial redox plays a critical role in the paradoxical effects of NAPDH oxidase-derived ROS on coronary endothelium.Cardiovascular research · 2017Article
- Calpain inhibition modulates glycogen synthase kinase 3β pathways in ischemic myocardium: A proteomic and mechanistic analysis.The Journal of thoracic and cardiovascular surgery · 2017Article
- NADPH Oxidase Deficiency: A Multisystem Approach.Oxidative medicine and cellular longevity · 2017Review
- Calpains and Coronary Vascular Disease.Circulation journal : official journal of the Japanese Circulation Society · 2016Review
- Involvement of NADPH oxidase in A2A adenosine receptor-mediated increase in coronary flow in isolated mouse hearts.Purinergic signalling · 2015Article
- The biphasic effects of oxidized-low density lipoprotein on the vasculogenic function of endothelial progenitor cells.PloS one · 2015Article
- Antioxidant Therapy: Is it your Gateway to Improved Cardiovascular Health?Pharmaceutica analytica acta · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
objectiveTo determine the functional significance of physiological reactive oxygen species (ROS) levels in endothelium-dependent nitric oxide (NO)-mediated coronary vasodilatation. METHODS AND
resultsEndothelium-derived NO is important in regulating coronary vascular tone. Excess ROS have been shown to reduce NO bioavailability, resulting in endothelial dysfunction and coronary diseases. NADPH oxidase is a major source of ROS in endothelial cells (ECs). By using lucigenin-based superoxide production and dichlorfluorescein diacetate (DCFH-DA) fluorescence-activated cell sorter assays, we found that mouse heart ECs from NADPH oxidase-knockdown (p47(phox-/-)) animals have reduced NADPH oxidase activity (>40%) and ROS levels (>30%) compared with wild-type mouse heart ECs. Surprisingly, a reduction in ROS did not improve coronary vasomotion; rather, endothelium-dependent vascular endothelial growth factor-mediated coronary vasodilatation was reduced by greater than 50% in p47(phox-/-) animals. Western blots and L-citrulline assays showed a significant reduction in Akt/protein kinase B (PKB) and endothelial NO synthase phosphorylation and NO synthesis, respectively, in p47(phox-/-) coronary vessels and mouse heart ECs. Adenoviral expression of constitutively active endothelial NO synthase restored vascular endothelial growth factor-mediated coronary vasodilatation in p47(phox-/-) animals.
conclusionsEndothelium-dependent vascular endothelial growth factor regulation of coronary vascular tone may require NADPH oxidase-derived ROS to activate phosphatidylinositol 3-kinase-Akt-endothelial NO synthase axis.
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