ArticleJournal of clinical microbiology2010
Rapid molecular identification of pathogenic yeasts by pyrosequencing analysis of 35 nucleotides of internal transcribed spacer 2.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Rapid identification of the Candida glabrata species complex by high-resolution melting curve analysis.Journal of clinical laboratory analysis · 2020Article
- Fluconazole Resistance in Isolates of Uncommon Pathogenic Yeast Species from the United Kingdom.Antimicrobial agents and chemotherapy · 2019Article
- Article
- Rapid identification of medically important Candida isolates using high resolution melting analysis.PloS one · 2015Article
- Development of two molecular approaches for differentiation of clinically relevant yeast species closely related to Candida guilliermondii and Candida famata.Journal of clinical microbiology · 2014Article
- Molecular identification of clinical "difficult-to-identify" microbes from sequencing 16S ribosomal DNA and internal transcribed spacer 2.Annals of clinical microbiology and antimicrobials · 2014Article
- Phylogenetic relationships matter: antifungal susceptibility among clinically relevant yeasts.Antimicrobial agents and chemotherapy · 2014Article
- Epidemiology, antifungal susceptibility, and pathogenicity of Candida africana isolates from the United Kingdom.Journal of clinical microbiology · 2013Article
- Characterization of a new clinical yeast species, Candida tunisiensis sp. nov., isolated from a strain collection from Tunisian hospitals.Journal of clinical microbiology · 2013Article
- Barcode identifiers as a practical tool for reliable species assignment of medically important black yeast species.Journal of clinical microbiology · 2012Article
- Laboratory diagnostics of invasive fungal infections: an overview with emphasis on molecular approach.Folia microbiologica · 2012Review
- Assessment of accuracy of identification of pathogenic yeasts in microbiology laboratories in the United kingdom.Journal of clinical microbiology · 2012Article
- Molecular identification and antifungal susceptibility testing of clinical isolates of the Candida rugosa species complex and proposal of the new species Candida neorugosa.Journal of clinical microbiology · 2012Article
- Evaluation of the commercial rapid trehalose test (GLABRATA RTT) for the point of isolation identification of Candida glabrata isolates in primary cultures.Mycopathologia · 2012Article
- Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2012Review
- Droplet-based pyrosequencing using digital microfluidics.Analytical chemistry · 2011Article
- Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Rapid identification of yeast species isolates from clinical samples is particularly important given their innately variable antifungal susceptibility profiles. Here, we have evaluated the utility of pyrosequencing analysis of a portion of the internal transcribed spacer 2 region (ITS2) for identification of pathogenic yeasts. A total of 477 clinical isolates encompassing 43 different fungal species were subjected to pyrosequencing analysis in a strictly blinded study. The molecular identifications produced by pyrosequencing were compared with those obtained using conventional biochemical tests (AUXACOLOR2) and following PCR amplification and sequencing of the D1-D2 portion of the nuclear 28S large rRNA gene. More than 98% (469/477) of isolates encompassing 40 of the 43 fungal species tested were correctly identified by pyrosequencing of only 35 bp of ITS2. Moreover, BLAST searches of the public synchronized databases with the ITS2 pyrosequencing signature sequences revealed that there was only minimal sequence redundancy in the ITS2 under analysis. In all cases, the pyrosequencing signature sequences were unique to the yeast species (or species complex) under investigation. Finally, when pyrosequencing was combined with the Whatman FTA paper technology for the rapid extraction of fungal genomic DNA, molecular identification could be accomplished within 6 h from the time of starting from pure cultures.
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