ArticleAmerican journal of physiology. Renal physiology2010
Nucleofection disrupts tight junction fence function to alter membrane polarity of renal epithelial cells.
Article in American journal of physiology. Renal physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 15 citations in OpenAlex.
- Improved alpharetrovirus-based Gag.MS2 particles for efficient and transient delivery of CRISPR-Cas9 into target cells.Molecular therapy. Nucleic acids · 2022Article
- The cellular response to plasma membrane disruption for nanomaterial delivery.Nano convergence · 2022Review
- Transient Retrovirus-Based CRISPR/Cas9 All-in-One Particles for Efficient, Targeted Gene Knockout.Molecular therapy. Nucleic acids · 2018Article
- Optimization of cerebellar purkinje neuron cultures and development of a plasmid-based method for purkinje neuron-specific, miRNA-mediated protein knockdown.Methods in cell biology · 2016Article
- TBC1D9B functions as a GTPase-activating protein for Rab11a in polarized MDCK cells.Molecular biology of the cell · 2014Article
- The role of epithelial tight junctions involved in pathogen infections.Molecular biology reports · 2014Review
- Multiple motifs regulate apical sorting of p75 via a mechanism that involves dimerization and higher-order oligomerization.Molecular biology of the cell · 2013Article
- Claudins and the modulation of tight junction permeability.Physiological reviews · 2013Review
- Physical non-viral gene delivery methods for tissue engineering.Annals of biomedical engineering · 2013Review
- Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism.Molecular biology of the cell · 2012Article
- OCRL1 modulates cilia length in renal epithelial cells.Traffic (Copenhagen, Denmark) · 2012Article
- Multiple biosynthetic trafficking routes for apically secreted proteins in MDCK cells.Traffic (Copenhagen, Denmark) · 2012Article
- Nucleofection induces non-specific changes in the metabolic activity of transfected cells.Molecular biology reports · 2012Article
- Core-glycosylated mucin-like repeats from MUC1 are an apical targeting signal.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Here, we compared the effects of nucleofection and lipid-based approaches to introduce siRNA duplexes on the subsequent development of membrane polarity in kidney cells. Nucleofection of Madin-Darby canine kidney (MDCK) cells, even with control siRNA duplexes, disrupted the initial surface polarity as well as the steady-state distribution of membrane proteins. Transfection using lipofectamine yielded slightly less efficient knockdown but did not disrupt membrane polarity. Polarized secretion was unaffected by nucleofection, suggesting a selective defect in the development of membrane polarity. Cilia frequency and length were not altered by nucleofection. However, the basolateral appearance of a fluorescent lipid tracer added to the apical surface of nucleofected cells was dramatically enhanced relative to untransfected controls or lipofectamine-treated cells. In contrast, [(3)H]inulin diffusion and transepithelial electrical resistance were not altered in nucleofected cells compared with untransfected ones. We conclude that nucleofection selectively hinders development of the tight junction fence function in MDCK cells.
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