ArticleToxicological sciences : an official journal of the Society of Toxicology2010
Comparison of the toxicity of smoke from conventional and harm reduction cigarettes using human embryonic stem cells.
Article in Toxicological sciences : an official journal of the Society of Toxicology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- Establishing a Disease-in-a-Dish Model to Study SARS-CoV-2 Infection During Prenatal Development.Current protocols · 2023Article
- Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics.Stem cells translational medicine · 2021Article
- A preview of selected articles-September 2021.Stem cells translational medicine · 2021Article
- Human embryonic stem cell classification: random network with autoencoded feature extractor.Journal of biomedical optics · 2021Article
- Effects of nicotine on the translation of stem cell therapy.Regenerative medicine · 2020Review
- Article
- Article
- DeephESC 2.0: Deep Generative Multi Adversarial Networks for improving the classification of hESC.PloS one · 2019Article
- Environmental Benzopyrene Attenuates Stemness of Placenta-Derived Mesenchymal Stem Cells via Aryl Hydrocarbon Receptor.Stem cells international · 2019Article
- Prospects and Frontiers of Stem Cell Toxicology.Stem cells and development · 2017Review
- Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy.Biomedical optics express · 2016Article
- Ambient particulate matter exposure and cardiovascular diseases: a focus on progenitor and stem cells.Journal of cellular and molecular medicine · 2016Review
- Thirdhand smoke: Chemical dynamics, cytotoxicity, and genotoxicity in outdoor and indoor environments.Toxicology in vitro : an international journal published in association with BIBRA · 2016Article
- Evaluating Cell Processes, Quality, and Biomarkers in Pluripotent Stem Cells Using Video Bioinformatics.PloS one · 2016Article
- Ambient Fine Particulate Matter Suppresses In Vivo Proliferation of Bone Marrow Stem Cells through Reactive Oxygen Species Formation.PloS one · 2015Article
- Cardiac development in zebrafish and human embryonic stem cells is inhibited by exposure to tobacco cigarettes and e-cigarettes.PloS one · 2015Article
- Lung deposition analyses of inhaled toxic aerosols in conventional and less harmful cigarette smoke: a review.International journal of environmental research and public health · 2013Review
- Potential for the embryonic morphogen Nodal as a prognostic and predictive biomarker in breast cancer.Breast cancer research : BCR · 2012Article
- Present state and future perspectives of using pluripotent stem cells in toxicology research.Archives of toxicology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
This study evaluated the hypothesis that smoke from harm reduction cigarettes impedes attachment and proliferation of H9 human embryonic stem cells (hESCs). Smoke from three harm reduction brands was compared with smoke from a conventional brand. Doses of smoke were measured in puff equivalents (PE) (1 PE = the amount of smoke in one puff that dissolves in 1 ml of medium). Cytotoxic doses were determined using morphological criteria and trypan blue staining, and apoptosis was confirmed using Magic Red staining. Attachment and proliferation of hESC were followed at a noncytotoxic dose in time-lapse videos collected using BioStation technology. Data were mined from videos either manually or using video bioinformatics subroutines developed with CL-Quant software. Mainstream (MS) and sidestream (SS) smoke from conventional and harm reduction cigarettes induced apoptosis in hESC colonies at 1 PE. At 0.1 PE (noncytotoxic), SS smoke from all brands inhibited attachment of hESC colonies to Matrigel with the strongest inhibition occurring in harm reduction brands. At 0.1 PE, SS smoke, but not MS smoke, from all brands inhibited hESC growth, and two harm reduction brands were more potent than the conventional brand. In general, hESC appeared more sensitive to smoke than their mouse ESC counterparts. Although harm reduction cigarettes are often marketed as safer than conventional brands, our assays show that SS smoke from harm reduction cigarettes was at least as potent or in some cases more potent than smoke from a conventional brand and that SS smoke was more inhibitory than MS smoke in all assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.