ArticleThe Journal of biological chemistry2010
The p97 ATPase dislocates MHC class I heavy chain in US2-expressing cells via a Ufd1-Npl4-independent mechanism.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- NPLOC4 Constructs Tumor Immunosuppressive Microenvironment in Pan-cancer and Hepatocellular Carcinoma.Current medicinal chemistry · 2026Article
- HCMV US2 co-opts TRC8 to degrade the endoplasmic reticulum-resident protein LMAN2L.The Journal of general virology · 2024Article
- Mechanisms of substrate processing during ER-associated protein degradation.Nature reviews. Molecular cell biology · 2023Review
- How Viruses Use the VCP/p97 ATPase Molecular Machine.Viruses · 2021Review
- The UFM1 Pathway Impacts HCMV US2-Mediated Degradation of HLA Class I.Molecules (Basel, Switzerland) · 2021Article
- Battle between Host Immune Cellular Responses and HCMV Immune Evasion.International journal of molecular sciences · 2019Review
- Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation.Journal of cell science · 2017Article
- The VCP/p97 and YOD1 Proteins Have Different Substrate-dependent Activities in Endoplasmic Reticulum-associated Degradation (ERAD).The Journal of biological chemistry · 2015Article
- Cyclophilin C Participates in the US2-Mediated Degradation of Major Histocompatibility Complex Class I Molecules.PloS one · 2015Article
- Degradation of the deubiquitinating enzyme USP33 is mediated by p97 and the ubiquitin ligase HERC2.The Journal of biological chemistry · 2014Article
- Diverse immune evasion strategies by human cytomegalovirus.Immunologic research · 2012Review
- Protein quality control in the ER: balancing the ubiquitin checkbook.Trends in cell biology · 2012Review
- Requirements for mouse mammary tumor virus Rem signal peptide processing and function.Journal of virology · 2012Article
- The cytoplasmic domain of rhesus cytomegalovirus Rh178 interrupts translation of major histocompatibility class I leader peptide-containing proteins prior to translocation.Journal of virology · 2011Article
- Protein folding and quality control in the endoplasmic reticulum: Recent lessons from yeast and mammalian cell systems.Current opinion in cell biology · 2011Review
- A ubiquitin ligase-associated chaperone holdase maintains polypeptides in soluble states for proteasome degradation.Molecular cell · 2011Article
- Proteostasis regulation at the endoplasmic reticulum: a new perturbation site for targeted cancer therapy.Cell research · 2011Review
- The complexities of p97 function in health and disease.Molecular bioSystems · 2011Review
- The AAA-ATPase p97 is essential for outer mitochondrial membrane protein turnover.Molecular biology of the cell · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The human cytomegalovirus (HCMV) protein US2 hijacks the endoplasmic reticulum (ER)-associated degradation machinery to dispose of MHC class I heavy chain (HC) at the ER. This process requires retrotranslocation of newly synthesized HC molecules from the ER membrane into the cytosol, but the mechanism underlying the dislocation reaction has been elusive. Here we establish an in vitro permeabilized cell assay that recapitulates the retrotranslocation of MHC HC in US2-expressing cells. Using this assay, we demonstrate that the dislocation process requires ATP and ubiquitin, as expected. The retrotranslocation also involves the p97 ATPase. However, the mechanism by which p97 dislocates MHC class I HC in US2 cells is distinct from that in US11 cells: the dislocation reaction in US2 cells is independent of the p97 cofactor Ufd1-Npl4. Our results suggest that different retrotranslocation mechanisms can employ distinct p97 ATPase complexes to dislocate substrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.