ArticleGenetica2010
Nuclear copies of mitochondrial genes: another problem for ancient DNA.
Article in Genetica, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed.
- Large variance in inbreeding within the Iberian wolf population.The Journal of heredity · 2024Article
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- Using loop-primer mediated PCR to enhance the detection of poorly preserved DNA.Frontiers in genetics · 2022Article
- Sharp Increase of Problematic Mitogenomes of Birds: Causes, Consequences, and Remedies.Genome biology and evolution · 2021Article
- New Insights Into Mitochondrial DNA Reconstruction and Variant Detection in Ancient Samples.Frontiers in genetics · 2021Article
- Molecular identification of Mazama species (Cervidae: Artiodactyla) from natural history collections.Genetics and molecular biology · 2020Article
- Evolutionary history of endemic Sulawesi squirrels constructed from UCEs and mitogenomes sequenced from museum specimens.BMC evolutionary biology · 2016Article
- Odintifier--A computational method for identifying insertions of organellar origin from modern and ancient high-throughput sequencing data based on haplotype phasing.BMC bioinformatics · 2015Article
- Pet fur or fake fur? A forensic approach.Investigative genetics · 2014Article
- Article
- Reconstructing the history of a fragmented and heavily exploited red deer population using ancient and contemporary DNA.BMC evolutionary biology · 2012Article
- Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of ancient DNA techniques is subject to many problems caused primarily by low quality and by low quantity of DNA. For these reasons most studies employing ancient DNA rely on the characterization of mitochondrial DNA, which is present in many more copies per cell than nuclear DNA and hence more copies are likely to survive. We used universal and taxon specific mitochondrial primers to amplify DNA from museum specimens, and found many instances where the amplification of nuclear copies of the mitochondrial gene (numts) instead of the targeted mitochondrial fragment had occurred. Furthermore, the likelihood of amplifying numts increased dramatically when universal primers were utilized. Here we suggest that ancient DNA practitioners must consider the possibility that numts can be amplified at higher rates than previously thought. This is another complication for ancient DNA studies, but it also suggests that more extensive inclusion of nuclear markers in ancient DNA studies should be feasible.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.