ArticlePLoS pathogens2010
Spliced leader trapping reveals widespread alternative splicing patterns in the highly dynamic transcriptome of Trypanosoma brucei.
Article in PLoS pathogens, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 123 papers.
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123 citing papers in PubMed.
- SF3B1 phosphorylation is an evolutionarily conserved step in spliceosome activation carried out by the divergent, OTS964-insensitive kinase CRK9 in trypanosomes.Nucleic acids research · 2026Article
- The UTRs of Leishmania donovani vary in length and are enriched in potential regulatory structures.PLoS pathogens · 2026Article
- Nanopore-mFrontiers in genetics · 2026Article
- DRBD3 regulates long non-coding RNA abundance and cryptic splice site selection in trypanosomes.Cellular and molecular life sciences : CMLS · 2025Article
- Evolution of SL-RNA Genes and Their Splicing Targets in Parasitic Flatworms.Molecular biology and evolution · 2025Article
- The UTRs of Leishmania donovani vary in length and are enriched in potential regulatory structures.bioRxiv : the preprint server for biology · 2025Article
- Exploring the activity of the putative Δ6-desaturase and its role in bloodstream form life-cycle transitions in Trypanosoma brucei.PLoS pathogens · 2025Article
- Differential regulatory role of AU-rich and GU-rich elements inFrontiers in microbiology · 2025Article
- Article
- Chemical Inhibition of Bromodomain Proteins in Insect-Stage African Trypanosomes Perturbs Silencing of the Variant Surface Glycoprotein Repertoire and Results in Widespread Changes in the Transcriptome.Microbiology spectrum · 2023Article
- The RNA export factor TbMex67 connects transcription and RNA export in Trypanosoma brucei and sets boundaries for RNA polymerase I.Nucleic acids research · 2023Article
- Aminoacyl tRNA Synthetases: Implications of Structural Biology in Drug Development against Trypanosomatid Parasites.ACS omega · 2023Review
- slORFfinder: a tool to detect open reading frames resulting from trans-splicing of spliced leader sequences.Briefings in bioinformatics · 2023Article
- Synthesis and characterization of I-BET151 derivatives for use in identifying protein targets in the African trypanosome.Current research in chemical biology · 2023Article
- Sequences and proteins that influence mRNA processing in Trypanosoma brucei: Evolutionary conservation of SR-domain and PTB protein functions.PLoS neglected tropical diseases · 2022Article
- TheRNA (New York, N.Y.) · 2022Article
- A diversified and segregated mRNA spliced-leader system in the parasitic Perkinsozoa.Open biology · 2022Article
- Article
- NNature · 2022Article
- Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.Frontiers in cellular and infection microbiology · 2022Review
63 more citing papers are in PubMed but not listed here.
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8 authors.
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Abstract
Trans-splicing of leader sequences onto the 5'ends of mRNAs is a widespread phenomenon in protozoa, nematodes and some chordates. Using parallel sequencing we have developed a method to simultaneously map 5'splice sites and analyze the corresponding gene expression profile, that we term spliced leader trapping (SLT). The method can be applied to any organism with a sequenced genome and trans-splicing of a conserved leader sequence. We analyzed the expression profiles and splicing patterns of bloodstream and insect forms of the parasite Trypanosoma brucei. We detected the 5' splice sites of 85% of the annotated protein-coding genes and, contrary to previous reports, found up to 40% of transcripts to be differentially expressed. Furthermore, we discovered more than 2500 alternative splicing events, many of which appear to be stage-regulated. Based on our findings we hypothesize that alternatively spliced transcripts present a new means of regulating gene expression and could potentially contribute to protein diversity in the parasite. The entire dataset can be accessed online at TriTrypDB or through: http://splicer.unibe.ch/.
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