ArticlePlant physiology2010
A gain-of-function mutation in the Arabidopsis disease resistance gene RPP4 confers sensitivity to low temperature.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers, 1 of them a synthesis that pooled it.
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Who cites it
52 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Meta-Analysis Reveals Opposite Effects of Biotic and Abiotic Stresses on Transcript Levels of Arabidopsis Intracellular Immune Receptor Genes.Frontiers in plant science · 2021Pooled it
- Natural Variation of a Specific NLR Gene RGA4L Confers Strong Chilling Tolerance in Rice.Plant biotechnology journal · 2025Article
- Pattern Recognition Receptors in Plant Immunity.Advances in experimental medicine and biology · 2025Review
- CsWRKY29, a key transcription factor in tea plant for freezing tolerance, ABA sensitivity, and sugar metabolism.Scientific reports · 2024Article
- INDUCER OF CBF EXPRESSION 1 promotes cold-enhanced immunity by directly activating salicylic acid signaling.The Plant cell · 2024Article
- Identification of genetic loci and candidate genes underlying freezing tolerance in wheat seedlings.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Broader functions of TIR domains in Arabidopsis immunity.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- An atypical NLR protein modulates the NRC immune receptor network in Nicotiana benthamiana.PLoS genetics · 2023Article
- The N-terminally truncated helper NLR NRG1C antagonizes immunity mediated by its full-length neighbors NRG1A and NRG1B.The Plant cell · 2022Article
- Plant autoimmunity-fresh insights into an old phenomenon.Plant physiology · 2022Article
- Interplay between CaInternational journal of molecular sciences · 2022Article
- Identification of multiple novel genetic mechanisms that regulate chilling tolerance in Arabidopsis.Frontiers in plant science · 2022Article
- spn-A/rad51 mutant exhibits enhanced genomic damage, cell death and low temperature sensitivity in somatic tissues.Chromosoma · 2021Article
- Natural variation in temperature-modulated immunity uncovers transcription factor bHLH059 as a thermoresponsive regulator in Arabidopsis thaliana.PLoS genetics · 2021Article
- Plant E3 ligases SNIPER1 and SNIPER2 broadly regulate the homeostasis of sensor NLR immune receptors.The EMBO journal · 2020Article
- Plant NLRs: The Whistleblowers of Plant Immunity.Plant communications · 2020Review
- SgRVE6, a LHY-CCA1-Like Transcription Factor From Fine-Stem Stylo, Upregulates NB-LRR Gene Expression and Enhances Cold Tolerance in Tobacco.Frontiers in plant science · 2020Article
- Responses of Manila Grass (Zoysia matrella) to chilling stress: From transcriptomics to physiology.PloS one · 2020Article
- BRASSINOSTEROID-INSENSITIVE2 Negatively Regulates the Stability of Transcription Factor ICE1 in Response to Cold Stress in Arabidopsis.The Plant cell · 2019Article
- Mutation of a Nucleotide-Binding Leucine-Rich Repeat Immune Receptor-Type Protein Disrupts Immunity to Bacterial Blight.Plant physiology · 2019Article
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5 authors.
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Abstract
How plants adapt to low temperature is not well understood. To identify components involved in low-temperature signaling, we characterized the previously isolated chilling-sensitive2 mutant (chs2) of Arabidopsis (Arabidopsis thaliana). This mutant grew normally at 22°C but showed phenotypes similar to activation of defense responses when shifted to temperatures below 16°C. These phenotypes include yellowish and collapsed leaves, increased electrolyte leakage, up-regulation of PATHOGENESIS RELATED genes, and accumulation of excess hydrogen peroxide and salicylic acid (SA). Moreover, the chs2 mutant was seedling lethal when germinated at or shifted for more than 3 d to low temperatures of 4°C to 12°C. Map-based cloning revealed that a single amino acid substitution occurred in the TIR-NB-LRR (for Toll/Interleukin-1 receptor- nucleotide-binding Leucine-rich repeat)-type resistance (R) protein RPP4 (for Recognition of Peronospora parasitica4), which causes a deregulation of the R protein in a temperature-dependent manner. The chs2 mutation led to an increase in the mutated RPP4 mRNA transcript, activation of defense responses, and an induction of cell death at low temperatures. In addition, a chs2 intragenic suppressor, in which the mutation occurs in the conserved NB domain, abolished defense responses at lower temperatures. Genetic analyses of chs2 in combination with known SA pathway and immune signaling mutants indicate that the chs2-conferred temperature sensitivity requires ENHANCED DISEASE SUSCEPTIBILITY1, REQUIRED FOR Mla12 RESISTANCE, and SUPPRESSOR OF G2 ALLELE OF skp1 but does not require PHYTOALEXIN DEFICIENT4, NONEXPRESSOR OF PR GENES1, or SA. This study reveals that an activated TIR-NB-LRR protein has a large impact on temperature sensitivity in plant growth and survival.
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