ArticleThe Journal of biological chemistry2010
A novel pax-like protein involved in transcriptional activation of cyst wall protein genes in Giardia lamblia.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Review
- Review
- A myeloid leukemia factor homolog is involved in tolerance to stresses and stress-induced protein metabolism in Giardia lamblia.Biology direct · 2023Article
- Identification of target genes regulated by encystation-induced transcription factor Myb2 using knockout mutagenesis in Giardia lamblia.Parasites & vectors · 2022Article
- A cell-cycle-dependent GARP-like transcriptional repressor regulates the initiation of differentiation inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Novel Spo11 Homologue Functions as a Positive Regulator in Cyst Differentiation inInternational journal of molecular sciences · 2021Article
- A Novel Multiprotein Bridging Factor 1-Like Protein Induces Cyst Wall Protein Gene Expression and Cyst Differentiation inInternational journal of molecular sciences · 2021Article
- New Genes Born-In or Invading Vertebrate Genomes.Frontiers in cell and developmental biology · 2021Review
- DNA topoisomerase IIIβ promotes cyst generation by inducing cyst wall protein gene expression inOpen biology · 2020Article
- Paired Box-1 (PAX1) Activates Multiple Phosphatases and Inhibits Kinase Cascades in Cervical Cancer.Scientific reports · 2019Article
- Article
- Homeodomain proteins: an update.Chromosoma · 2016Review
- The genome and transcriptome of the enteric parasite Entamoeba invadens, a model for encystation.Genome biology · 2013Article
- DNA topoisomerase II is involved in regulation of cyst wall protein genes and differentiation in Giardia lamblia.PLoS neglected tropical diseases · 2013Article
- Functional redundancy of two Pax-like proteins in transcriptional activation of cyst wall protein genes in Giardia lamblia.PloS one · 2012Article
- A microRNA derived from an apparent canonical biogenesis pathway regulates variant surface protein gene expression in Giardia lamblia.RNA (New York, N.Y.) · 2011Article
- A novel E2F-like protein involved in transcriptional activation of cyst wall protein genes in Giardia lamblia.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Giardia lamblia differentiates into infectious cysts to survive outside of the host. It is of interest to identify factors involved in up-regulation of cyst wall proteins (CWPs) during this differentiation. Pax proteins are important regulators of development and cell differentiation in Drosophila and vertebrates. No member of this gene family has been reported to date in yeast, plants, or protozoan parasites. We have identified a pax-like gene (pax1) encoding a putative paired domain in the G. lamblia genome. Epitope-tagged Pax1 localized to nuclei during both vegetative growth and encystation. Recombinant Pax1 specifically bound to the AT-rich initiator elements of the encystation-induced cwp1 to -3 and myb2 genes. Interestingly, overexpression of Pax1 increased cwp1 to -3 and myb2 gene expression and cyst formation. Deletion of the C-terminal paired domain or mutation of the basic amino acids of the paired domain resulted in a decrease of the transactivation function of Pax1. Our results indicate that the Pax family has been conserved during evolution, and Pax1 could up-regulate the key encystation-induced genes to regulate differentiation of the protozoan eukaryote, G. lamblia.
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