ArticleMethods in enzymology2010
Mouse mutagenesis with the chemical supermutagen ENU.
Article in Methods in enzymology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Critical roles of MCM8 in meiotic recombination during mouse spermatogenesis.bioRxiv : the preprint server for biology · 2026Article
- Intercellular bridges are essential for transposon repression and meiosis in the male germline.Nature communications · 2025Article
- Technologies to Study Genetics and Molecular Pathways.Advances in experimental medicine and biology · 2024Review
- shani mutation in mouse affects splicing of Spata22 and leads to impaired meiotic recombination.Chromosoma · 2020Article
- An ENU-induced mutation in Twist1 transactivation domain causes hindlimb polydactyly with complete penetrance and dominant-negatively impairs E2A-dependent transcription.Scientific reports · 2020Article
- Predicting human disease mutations and identifying drug targets from mouse gene knockout phenotyping campaigns.Disease models & mechanisms · 2019Review
- A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe.Journal of visualized experiments : JoVE · 2019Article
- Article
- rahu is a mutant allele of Dnmt3c, encoding a DNA methyltransferase homolog required for meiosis and transposon repression in the mouse male germline.PLoS genetics · 2017Article
- The old and new face of craniofacial research: How animal models inform human craniofacial genetic and clinical data.Developmental biology · 2016Review
- Identification of a Novel ENU-Induced Mutation in MouseNeural plasticity · 2016Article
- Mouse ENU Mutagenesis to Understand Immunity to Infection: Methods, Selected Examples, and Perspectives.Genes · 2014Review
- The functional diversity of essential genes required for mammalian cardiac development.Genesis (New York, N.Y. : 2000) · 2014Review
- Characterization of two ENU-induced mutations affecting mouse skeletal morphology.G3 (Bethesda, Md.) · 2013Article
- Going forward with genetics: recent technological advances and forward genetics in mice.The American journal of pathology · 2013Review
- Deficiency of FRAS1-related extracellular matrix 1 (FREM1) causes congenital diaphragmatic hernia in humans and mice.Human molecular genetics · 2013Article
- Novel frem1-related mouse phenotypes and evidence of genetic interactions with gata4 and slit3.PloS one · 2013Article
- An allelic series of mice reveals a role for RERE in the development of multiple organs affected in chromosome 1p36 deletions.PloS one · 2013Article
- Chromosome substitution strains: gene discovery, functional analysis, and systems studies.Mammalian genome : official journal of the International Mammalian Genome Society · 2012Review
- Discovery Genetics - The History and Future of Spontaneous Mutation Research.Current protocols in mouse biology · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The generation and analysis of germline mutations in the mouse is one of the cornerstones of modern biological research. The chemical supermutagen N-ethyl-N-nitrosourea (ENU) is the most potent known mouse mutagen and can be used to generate point mutations throughout the mouse genome. The progeny of ENU-mutagenized males can be screened for autosomal dominant phenotypes, or they can be used to generate multigeneration pedigrees to screen for autosomal recessive traits. The introduction of balancer chromosomes into the breeding scheme can allow for the selective capture of mutations in a specific chromosomal region. More recent work has demonstrated that the use of animals that already have a mutation of interest can lead to the successful isolation of additional mutations that modify the original mutant phenotype. Further, modern molecular techniques ensure that mutations can be readily identified. We describe here the procedures for mutagenizing male mice with ENU and explain the various types of screens that can be performed for different kinds of induced mutations. The currently published research on ENU mutagenesis in the mouse has only scratched the surface of what is possible with this powerful technique, and further work is certain to deepen our knowledge of the role of the individual components of the mouse genome and the myriad relationships between them.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.