ArticleBMC cancer2010
Involvement of tumor necrosis factor-alpha in the upregulation of CXCR4 expression in gastric cancer induced by Helicobacter pylori.
Article in BMC cancer, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 62 citations in OpenAlex.
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- TNF-α Secreted from Macrophages Increases the Expression of Prometastatic Integrin αV in Gastric Cancer.International journal of molecular sciences · 2022Article
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- Peripheral Cytokine Levels as a Prognostic Indicator in Gastric Cancer: A Review of Existing Literature.Biomedicines · 2021Review
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- Role of CXCR4 as a Prognostic Biomarker Associated With the Tumor Immune Microenvironment in Gastric Cancer.Frontiers in cell and developmental biology · 2021Article
- Tumor-associated macrophages regulate gastric cancer cell invasion and metastasis through TGFβ2/NF-κB/Kindlin-2 axis.Chinese journal of cancer research = Chung-kuo yen cheng yen chiu · 2020Article
- Role of Obesity in the Tumorigenesis of Gastric Cancer.International journal of preventive medicine · 2020Review
- Tumor Necrosis Factor α Blockade: An Opportunity to Tackle Breast Cancer.Frontiers in oncology · 2020Review
- LPS-induced CXCR7 expression promotes gastric Cancer proliferation and migration via the TLR4/MD-2 pathway.Diagnostic pathology · 2019Article
- Inhibition of CXCL12/CXCR4 axis as a potential targeted therapy of advanced gastric carcinoma.Cancer medicine · 2017Review
- Status of Epstein-Barr Virus Coinfection withJournal of oncology · 2017Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundH. pylori, whose infection increases tumor invasiveness and metastasis, is generally labelled as the strongest risk factor for the development of gastric cancer. It appears not to be a coincidence that there is also an overexpression of CXCR4 and an obvious involvement in gastric cancer metastasis. The aim of this study attempts to investigate and further to establish a link between them. With H. pylori being a potent inducer of TNF-alpha, whether TNF-alpha, a tumor promoter, is involved in the induction of CXCR4 expression by H. pylori was also under research in this study.
methodsExpression of CXCR4, TNF-alpha, IL-6 and IL-1 beta mRNA was determined by real-time PCR. CXCR4 protein expression was detected by Western blotting. Concentrations of TNF-alpha, IL-6 and IL-1 beta in cell culture supernatants were measured using the Quantikine Elisa kit. To abrogate TNF-alpha expression in HGC27 cells, TNF-alpha RNAi plasmid was used to transfect them.
resultsLevels of CXCR4 and TNF-alpha mRNA were significantly higher in H. pylori-positive gastric cancers (n = 19) compared to H. pylori-negative ones (n = 15). A subsequently Spearman's rank correlation test showed there was a positive correlation between the level of CXCR4 mRNA and that of TNF-alpha in 34 primary gastric cancers. Other results followed: Expression of CXCR4 and TNF-alpha was upregulated in gastric cancer cell MKN45 and HGC27 after infection with H. pylori 26695 (cag PAI+ ) or Tx30a (cag PAI- ); The induction of CXCR4 expression by H. pylori was inhibited significantly by a neutralizing TNF-alpha antibody, infliximab; CXCR4 expression was upregulated in MKN45 cells after treatment with exogenous TNF-alpha or co-culture with macrophage, and was downregulated in HGC27 cells after transfection with TNF-alpha RNAi plasmid. There was a significant increase in the migration of MKN45 cells treated with H. pylori 26695, and a strong inhibition when AMD 3100, a CXCR4 antagonist, or infliximab, was added.
conclusionsOur findings demonstrated that H. pylori upregulates CXCR4 expression in gastric cancer through TNF-alpha.
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