ArticleBMC microbiology2010
The clinical Pseudomonas fluorescens MFN1032 strain exerts a cytotoxic effect on epithelial intestinal cells and induces Interleukin-8 via the AP-1 signaling pathway.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- N-glycans in liver-secreted and immunoglogulin-derived protein fractions.Journal of proteomics · 2012Trial
- Antibiotic-induced Malassezia expansion in the infant gut promotes early-life immune dysregulation and airway inflammation in mice.Nature communications · 2026Article
- Comparison of Different Sites of Microbiota Shows Increased Pseudomonas Species in the Gut Mucosa in Inflammatory Bowel Disease.Inflammatory bowel diseases · 2026Article
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- The regulator FleQ both transcriptionally and post-transcriptionally regulates the level of RTX adhesins ofJournal of bacteriology · 2023Article
- The Regulator FleQ Post-Transcriptionally Regulates the Production of RTX Adhesins bybioRxiv : the preprint server for biology · 2023Article
- Legionella and other opportunistic pathogens in full-scale chloraminated municipal drinking water distribution systems.Water research · 2021Article
- Interaction of human dynein light chain 1 (DYNLL1) with enterochelin esterase (Saudi journal of biological sciences · 2020Article
- Biofilm and Pathogenesis-Related Proteins in the FoodborneFrontiers in microbiology · 2020Article
- Draft Genome Sequence of Carbapenem-Resistant Pseudomonas fluorescens Strain BWKM6, Isolated from Feces of Mareca penelope.Genome announcements · 2018Article
- Comparative genomics of Pseudomonas fluorescens subclade III strains from human lungs.BMC genomics · 2015Article
- Microbiology, genomics, and clinical significance of the Pseudomonas fluorescens species complex, an unappreciated colonizer of humans.Clinical microbiology reviews · 2014Review
- Propensity to high-fat diet-induced obesity in mice is associated with the indigenous opportunistic bacteria on the interior of Peyer's patches.Journal of clinical biochemistry and nutrition · 2014Article
- Exploring the influence of the gut microbiota and probiotics on health: a symposium report.The British journal of nutrition · 2014Article
- Cell-associated bacteria in the human lung microbiome.Microbiome · 2014Article
- Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients.BMC microbiology · 2013Article
- A genetic approach to the development of new therapeutic phages to fight pseudomonas aeruginosa in wound infections.Viruses · 2012Review
- Virulence of the Pseudomonas fluorescens clinical strain MFN1032 towards Dictyostelium discoideum and macrophages in relation with type III secretion system.BMC microbiology · 2012Article
- Involvement of peptidylprolyl cis/trans isomerases in Enterococcus faecalis virulence.Infection and immunity · 2012Article
- Chitosan oligosaccharides suppress LPS-induced IL-8 expression in human umbilical vein endothelial cells through blockade of p38 and Akt protein kinases.Acta pharmacologica Sinica · 2011Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPseudomonas fluorescens is present in low number in the intestinal lumen and has been proposed to play a role in Crohn's disease (CD). Indeed, a highly specific antigen, I2, has been detected in CD patients and correlated to the severity of the disease. We aimed to determine whether P. fluorescens was able to adhere to human intestinal epithelial cells (IECs), induce cytotoxicity and activate a proinflammatory response.
resultsBehaviour of the clinical strain P. fluorescens MFN1032 was compared to that of the psychrotrophic strain P. fluorescens MF37 and the opportunistic pathogen P. aeruginosa PAO1. Both strains of P. fluorescens were found to adhere on Caco-2/TC7 and HT-29 cells. Their cytotoxicity towards these two cell lines determined by LDH release assays was dose-dependent and higher for the clinical strain MFN1032 than for MF37 but lower than P. aeruginosa PAO1. The two strains of P. fluorescens also induced IL-8 secretion by Caco-2/TC7 and HT-29 cells via the AP-1 signaling pathway whereas P. aeruginosa PAO1 potentially used the NF-kappaB pathway.
conclusionsThe present work shows, for the first time, that P. fluorescens MFN1032 is able to adhere to IECs, exert cytotoxic effects and induce a proinflammatory reaction. Our results are consistent with a possible contribution of P. fluorescens in CD and could explain the presence of specific antibodies against this bacterium in the blood of patients.
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