ArticleBMC genomics2010
A complete DNA sequence map of the ovine major histocompatibility complex.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Structural Prediction and Antigenic Characterization of Recombinant Nucleocapsid Protein (p14) of Small Ruminant Lentivirus.Viruses · 2026Article
- Whole genome copy number variation analysis to detect genomic regions for resistance to Haemonchus contortus in Sheep.BMC genomics · 2026Article
- Genome-wide association with footrot in hair and wool sheep.Frontiers in genetics · 2023Article
- MHC class IIa haplotypes derived by high-throughput SNP screening in an isolated sheep population.G3 (Bethesda, Md.) · 2021Article
- A chromosome-scale reference genome and genome-wide genetic variations elucidate adaptation in yak.Molecular ecology resources · 2021Article
- Characterization of two MHC II genes (DOB, DRB) in white-tailed deer (Odocoileus virginianus).BMC genetics · 2020Article
- Allelic nomenclature for the duplicated MHC class II DQ genes in sheep.Immunogenetics · 2019Article
- Accuracy of imputation to whole-genome sequence in sheep.Genetics, selection, evolution : GSE · 2019Article
- Chromosome-Level Alpaca Reference GenomeFrontiers in genetics · 2019Article
- Structural and functional diversity arising from intra- and inter-haplotype combinations of duplicated DQA and B loci within the ovine MHC.Immunogenetics · 2018Article
- Insights into the ancestral organisation of the mammalian MHC class II region from the genome of the pteropid bat, Pteropus alecto.BMC genomics · 2017Article
- Ensembl core software resources: storage and programmatic access for DNA sequence and genome annotation.Database : the journal of biological databases and curation · 2017Article
- Organization and characteristics of the major histocompatibility complex class II region in the Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis).Scientific reports · 2016Article
- A comprehensive mapping of the structure and gene organisation in the sheep MHC class I region.BMC genomics · 2015Article
- Comparative genome analyses reveal distinct structure in the saltwater crocodile MHC.PloS one · 2014Article
- Sequencing and automated whole-genome optical mapping of the genome of a domestic goat (Capra hircus).Nature biotechnology · 2013Article
- Modified uterine allotransplantation and immunosuppression procedure in the sheep model.PloS one · 2013Article
- A physical map of a BAC clone contig covering the entire autosome insertion between ovine MHC Class IIa and IIb.BMC genomics · 2012Article
- Isolation of a 97-kb minimal essential MHC B locus from a new reverse-4D BAC library of the golden pheasant.PloS one · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe ovine Major Histocompatibility Complex (MHC) harbors clusters of genes involved in overall resistance/susceptibility of an animal to infectious pathogens. However, only a limited number of ovine MHC genes have been identified and no adequate sequence information is available, as compared to those of swine and bovine. We previously constructed a BAC clone-based physical map that covers entire class I, class II and class III region of ovine MHC. Here we describe the assembling of a complete DNA sequence map for the ovine MHC by shotgun sequencing of 26 overlapping BAC clones.
resultsDNA shotgun sequencing generated approximately 8-fold genome equivalent data that were successfully assembled into a finished sequence map of the ovine MHC. The sequence map spans approximately 2,434,000 nucleotides in length, covering almost all of the MHC loci currently known in the sheep and cattle. Gene annotation resulted in the identification of 177 protein-coding genes/ORFs, among which 145 were not previously reported in the sheep, and 10 were ovine species specific, absent in cattle or other mammals. A comparative sequence analyses among human, sheep and cattle revealed a high conservation in the MHC structure and loci order except for the class II, which were divided into IIa and IIb subregions in the sheep and cattle, separated by a large piece of non-MHC autosome of approximately 18.5 Mb. In addition, a total of 18 non-protein-coding microRNAs were predicted in the ovine MHC region for the first time.
conclusionAn ovine MHC DNA sequence map was successfully assembled by shotgun sequencing of 26 overlapping BAC clone. This makes the sheep the second ruminant species for which the complete MHC sequence information is available for evolution and functional studies, following that of the bovine. The results of the comparative analysis support a hypothesis that an inversion of the ancestral chromosome containing the MHC has shaped the MHC structures of ruminants, as we currently observed in the sheep and cattle. Identification of relative large numbers of microRNAs in the ovine MHC region helps to provide evidence that microRNAs are actively involved in the regulation of MHC gene expression and function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.