ArticleAnalytical chemistry2010
Minimizing tissue damage in electroosmotic sampling.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Electroosmotic Perfusion-Microdialysis Probe Created by Direct Laser Writing for Quantitative Assessment of Leucine Enkephalin Hydrolysis by Insulin-Regulated Aminopeptidase in Vivo.Analytical chemistry · 2020Article
- Electrokinetic infusions into hydrogels and brain tissue: Control of direction and magnitude of solute delivery.Journal of neuroscience methods · 2019Article
- Methods of Measuring Enzyme Activity Ex Vivo and In Vivo.Annual review of analytical chemistry (Palo Alto, Calif.) · 2018Review
- Higher Aminopeptidase Activity Determined by Electroosmotic Push-Pull Perfusion Contributes to Selective Vulnerability of the Hippocampal CA1 Region to Oxygen Glucose Deprivation.ACS chemical neuroscience · 2018Article
- Numerical Modeling of Electroosmotic Push-Pull Perfusion and Assessment of Its Application to Quantitative Determination of Enzymatic Activity in the Extracellular Space of Mammalian Tissue.Analytical chemistry · 2017Article
- Experimental evaluation and computational modeling of tissue damage from low-flow push-pull perfusion sampling in vivo.Journal of neuroscience methods · 2015Review
- Electroosmotic perfusion of tissue: sampling the extracellular space and quantitative assessment of membrane-bound enzyme activity in organotypic hippocampal slice cultures.Analytical and bioanalytical chemistry · 2014Review
- Integrated electroosmotic perfusion of tissue with online microfluidic analysis to track the metabolism of cystamine, pantethine, and coenzyme A.Analytical chemistry · 2013Article
- Stimulation and release from neurons via a dual capillary collection device interfaced to mass spectrometry.The Analyst · 2013Article
- Assessment of tissue viability following electroosmotic push-pull perfusion from organotypic hippocampal slice cultures.ACS chemical neuroscience · 2013Article
- Electroosmotic push-pull perfusion: description and application to qualitative analysis of the hydrolysis of exogenous galanin in organotypic hippocampal slice cultures.ACS chemical neuroscience · 2013Article
- An in situ measurement of extracellular cysteamine, homocysteine, and cysteine concentrations in organotypic hippocampal slice cultures by integration of electroosmotic sampling and microfluidic analysis.Analytical chemistry · 2013Article
- Iontophoresis from a micropipet into a porous medium depends on the ζ-potential of the medium.Analytical chemistry · 2012Article
- A simple method for measuring organotypic tissue slice culture thickness.Journal of neuroscience methods · 2011Article
- Minimizing tissue damage in electroosmotic sampling.Analytical chemistry · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Electroosmotic sampling is a potentially powerful method for pulling extracellular fluid into a fused-silica capillary in contact with the surface of tissue. An electric field is created in tissue by passing current through an electrolyte-filled capillary and then through the tissue. The resulting field acts on the counterions to the surface charges in the extracellular space to create electroosmotic fluid flow within the extracellular space of a tissue. Part of the development of this approach is to define conditions under which electroosmotic sampling minimizes damage to the tissue, in this case organotypic hippocampal slice cultures (OHSCs). We have assessed tissue damage by measuring fluorescence resulting from exposing sampled tissue to propidium iodide solution 16-24 h after sampling. Sampling has been carried out with a variety of capillary diameters, capillary tip-tissue distances, and applied voltages. Tissue damage is negligible when the power (current x potential drop) created in the tissue is less than 120 microW. In practical terms, smaller capillary i.d.s, lower voltages, and greater tissue to capillary distances lead to lower power.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.