ArticleThe Journal of membrane biology2010
Protein kinase C activation has distinct effects on the localization, phosphorylation and detergent solubility of the claudin protein family in tight and leaky epithelial cells.
Article in The Journal of membrane biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 36 citations in OpenAlex.
- Brazilian Green Propolis Rescues Oxidative Stress-Induced Mislocalization of Claudin-1 in Human Keratinocyte-Derived HaCaT Cells.International journal of molecular sciences · 2019Article
- Claudin 1 Is Highly Upregulated by PKC in MCF7 Human Breast Cancer Cells and Correlates Positively with PKCε in Patient Biopsies.Translational oncology · 2019Article
- Distinct Origin of Claudin7 in Early Tumor Endosomes Affects Exosome Assembly.International journal of biological sciences · 2019Article
- Ping-Pong-Tumor and Host in Pancreatic Cancer Progression.Frontiers in oncology · 2019Review
- A Promising Candidate: Heparin-Binding Protein Steps onto the Stage of Sepsis Prediction.Journal of immunology research · 2019Review
- Bicellular Tight Junctions and Wound Healing.International journal of molecular sciences · 2018Review
- Organic osmolytes preserve the function of the developing tight junction in ultraviolet B-irradiated rat epidermal keratinocytes.Scientific reports · 2018Article
- Infection with hepatitis C virus depends on TACSTD2, a regulator of claudin-1 and occludin highly downregulated in hepatocellular carcinoma.PLoS pathogens · 2018Article
- Tight Junction Proteins and Signaling Pathways in Cancer and Inflammation: A Functional Crosstalk.Frontiers in physiology · 2018Review
- Non-invasive saliva human biomonitoring: development of an in vitro platform.Journal of exposure science & environmental epidemiology · 2017Article
- The Role of miR-330-3p/PKC-α Signaling Pathway in Low-Dose Endothelial-Monocyte Activating Polypeptide-II Increasing the Permeability of Blood-Tumor Barrier.Frontiers in cellular neuroscience · 2017Article
- Pancreatic cancer stem cell markers and exosomes - the incentive push.World journal of gastroenterology · 2016Review
- Palmitoylated claudin7 captured in glycolipid-enriched membrane microdomains promotes metastasis via associated transmembrane and cytosolic molecules.Oncotarget · 2016Article
- The importance of claudin-7 palmitoylation on membrane subdomain localization and metastasis-promoting activities.Cell communication and signaling : CCS · 2015Article
- Claudin-7 promotes the epithelial-mesenchymal transition in human colorectal cancer.Oncotarget · 2015Article
- Changes in the phosphorylation of claudins during the course of experimental colitis.International journal of clinical and experimental pathology · 2015Article
- Stanniocalicin 2 suppresses breast cancer cell migration and invasion via the PKC/claudin-1-mediated signaling.PloS one · 2015Article
- Keratin 76 is required for tight junction function and maintenance of the skin barrier.PLoS genetics · 2014Article
- Regulation of paracellular permeability: factors and mechanisms.Molecular biology reports · 2013Review
- Keratin 8 and 18 loss in epithelial cancer cells increases collective cell migration and cisplatin sensitivity through claudin1 up-regulation.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously shown that protein kinase C (PKC) activation has distinct effects on the structure and barrier properties of cultured epithelial cells (HT29 and MDCK I). Since the claudin family of tight junction (TJ)-associated proteins is considered to be crucial for the function of mature TJ, we assessed their expression patterns and cellular destination, detergent solubility and phosphorylation upon PKC stimulation for 2 or 18 h with phorbol myristate acetate (PMA). In HT29 cells, claudins 1, 3, 4 and 5 and possibly claudin 2 were redistributed to apical cell-cell contacts after PKC activation and the amounts of claudins 1, 3 and 5, but not of claudin 2, were increased in cell lysates. By contrast, in MDCK I cells, PMA treatment resulted in redistribution of claudins 1, 3, 4 and 5 from the TJ and in reorganization of the proteins into more insoluble complexes. Claudins 1 and 4 were phosphorylated in both MDCK I and HT29 cells, but PKC-induced changes in claudin phosphorylation state were detected only in MDCK I cells. A major difference between HT29 and MDCK I cells, which have low and high basal transepithelial electrical resistance, respectively, was the absence of claudin 2 in the latter. Our findings show that PKC activation targets in characteristic ways the expression patterns, destination, detergent solubility and phosphorylation state of claudins in epithelial cells with different capacities to form an epithelial barrier.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.