ArticleThe Journal of biological chemistry2010
Rod/Zw10 complex is required for PIASy-dependent centromeric SUMOylation.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- dsRNAi-mediated silencing of PIAS2beta specifically kills anaplastic carcinomas by mitotic catastrophe.Nature communications · 2024Article
- The deubiquitinase USP36 promotes snoRNP group SUMOylation and is essential for ribosome biogenesis.EMBO reports · 2021Article
- PICH regulates the abundance and localization of SUMOylated proteins on mitotic chromosomes.Molecular biology of the cell · 2020Article
- Comprehensive analysis of 5-hydroxymethylcytosine in zw10 kinetochore protein as a promising biomarker for screening and diagnosis of early colorectal cancer.Clinical and translational medicine · 2020Article
- Reversible regulation of ORC2 SUMOylation by PIAS4 and SENP2.Oncotarget · 2017Article
- Identification of a new small ubiquitin-like modifier (SUMO)-interacting motif in the E3 ligase PIASy.The Journal of biological chemistry · 2017Article
- SUMOylation of DNA topoisomerase IIα regulates histone H3 kinase Haspin and H3 phosphorylation in mitosis.The Journal of cell biology · 2016Article
- Novel Role for Protein Inhibitor of Activated STAT 4 (PIAS4) in the Restriction of Herpes Simplex Virus 1 by the Cellular Intrinsic Antiviral Immune Response.Journal of virology · 2016Article
- SUMOylation-Mediated Regulation of Cell Cycle Progression and Cancer.Trends in biochemical sciences · 2015Review
- SUMOylation regulates polo-like kinase 1-interacting checkpoint helicase (PICH) during mitosis.The Journal of biological chemistry · 2015Article
- SUMOylation of the C-terminal domain of DNA topoisomerase IIα regulates the centromeric localization of Claspin.Cell cycle (Georgetown, Tex.) · 2015Article
- SUMOylation of Psmd1 controls Adrm1 interaction with the proteasome.Cell reports · 2014Article
- Moonlighting functions of the NRZ (mammalian Dsl1) complex.Frontiers in cell and developmental biology · 2014Review
- SUMO rules: regulatory concepts and their implication in neurologic functions.Neuromolecular medicine · 2013Review
- SUMO: a multifaceted modifier of chromatin structure and function.Developmental cell · 2013Review
- Article
- The SUMO ligase PIAS1 regulates UV-induced apoptosis by recruiting Daxx to SUMOylated foci.Journal of cell science · 2012Article
- SUMOylation in control of accurate chromosome segregation during mitosis.Current protein & peptide science · 2012Review
- Functions of the poly(ADP-ribose) polymerase superfamily in plants.Cellular and molecular life sciences : CMLS · 2012Review
- PIASy-dependent SUMOylation regulates DNA topoisomerase IIalpha activity.The Journal of cell biology · 2010Article
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Authors and funding
2 authors.
Funding
Abstract
SUMO conjugation of cellular proteins is essential for proper progression of mitosis. PIASy, a SUMO E3 ligase, is required for mitotic SUMOylation of chromosomal proteins, yet the regulatory mechanism behind the PIASy-dependent SUMOylation during mitosis has not been determined. Using a series of truncated PIASy proteins, we have found that the N terminus of PIASy is not required for SUMO modification in vitro but is essential for mitotic SUMOylation in Xenopus egg extracts. We demonstrate that swapping the N terminus of PIASy protein with the corresponding region of other PIAS family members abolishes chromosomal binding and mitotic SUMOylation. We further show that the N-terminal domain of PIASy is sufficient for centromeric localization. We identified that the N-terminal domain of PIASy interacts with the Rod/Zw10 complex, and immunofluorescence further reveals that PIASy colocalizes with Rod/Zw10 in the centromeric region. We show that the Rod/Zw10 complex interacts with the first 47 residues of PIASy which were particularly important for mitotic SUMOylation. Finally, we show that depletion of Rod compromises the centromeric localization of PIASy and SUMO2/3 in mitosis. Together, we demonstrate a fundamental mechanism of PIASy to localize in the centromeric region of chromosome to execute centromeric SUMOylation during mitosis.
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