ArticleBMC microbiology2010
Multi-locus analysis of human infective Cryptosporidium species and subtypes using ten novel genetic loci.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Genetic Diversity of Cryptosporidium in Children in an Urban Informal Settlement of Nairobi, Kenya.PloS one · 2015Trial
- Discovery of an Orally Efficacious Pyrazolo[3,4-Journal of medicinal chemistry · 2025Article
- Design, development, and testing of a new multi-locus sequence typing scheme for the zoonotic pathogenCurrent research in parasitology & vector-borne diseases · 2025Article
- Scalable cryopreservation of infectious Cryptosporidium hominis oocysts by vitrification.PLoS pathogens · 2023Article
- Fully resolved assembly of Cryptosporidium parvum.GigaScience · 2022Article
- Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.Frontiers in cellular and infection microbiology · 2022Review
- The prevalence and genetic characterisation ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Spontaneous Selection ofAntimicrobial agents and chemotherapy · 2021Article
- Giardia/Cryptosporidium QUIK CHEK Assay Is More Specific Than Quantitative Polymerase Chain Reaction for Rapid Point-of-care Diagnosis of Cryptosporidiosis in Infants in Bangladesh.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
- First detection of Cryptosporidium DNA in blood and cerebrospinal fluid of HIV-infected patients.Parasitology research · 2018Article
- Detection of Intestinal Pathogens in River, Shore, and Drinking Water in Lima, Peru.Journal of genomics · 2017Article
- Cryptosporidium infection in solid organ transplantation.World journal of transplantation · 2016Review
- Novel real-time PCR assays for the specific detection of human infective Cryptosporidium species.Virulence · 2016Article
- Subtyping of Cryptosporidium cuniculus and genotyping of Enterocytozoon bieneusi in rabbits in two farms in Heilongjiang Province, China.Parasite (Paris, France) · 2016Article
- Generation of whole genome sequences of new Cryptosporidium hominis and Cryptosporidium parvum isolates directly from stool samples.BMC genomics · 2015Article
- Oxidative stress control by apicomplexan parasites.BioMed research international · 2015Review
- A new heterogeneous family of telomerically encoded Cryptosporidium proteins.Evolutionary applications · 2013Article
- Cryptosporidium pathogenicity and virulence.Clinical microbiology reviews · 2013Review
- Article
- Cryptosporidium cuniculus and Giardia duodenalis in rabbits: genetic diversity and possible zoonotic transmission.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCryptosporidium is a protozoan parasite that causes diarrheal illness in a wide range of hosts including humans. Two species, C. parvum and C. hominis are of primary public health relevance. Genome sequences of these two species are available and show only 3-5% sequence divergence. We investigated this sequence variability, which could correspond either to sequence gaps in the published genome sequences or to the presence of species-specific genes. Comparative genomic tools were used to identify putative species-specific genes and a subset of these genes was tested by PCR in a collection of Cryptosporidium clinical isolates and reference strains.
resultsThe majority of the putative species-specific genes examined were in fact common to C. parvum and C. hominis. PCR product sequence analysis revealed interesting SNPs, the majority of which were species-specific. These genetic loci allowed us to construct a robust and multi-locus analysis. The Neighbour-Joining phylogenetic tree constructed clearly discriminated the previously described lineages of Cryptosporidium species and subtypes.
conclusionsMost of the genes identified as being species specific during bioinformatics in Cryptosporidium sp. are in fact present in multiple species and only appear species specific because of gaps in published genome sequences. Nevertheless SNPs may offer a promising approach to studying the taxonomy of closely related species of Cryptosporidia.
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