ArticleMolecular plant pathology2010
The Xanthomonas citri effector protein PthA interacts with citrus proteins involved in nuclear transport, protein folding and ubiquitination associated with DNA repair.
Article in Molecular plant pathology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 52 citations in OpenAlex.
- Article
- Nuclear effectors of plant pathogens: Distinct strategies to be one step ahead.Molecular plant pathology · 2023Review
- Transient expression of an scFvG8 antibody in plants and characterization of its effects on the virulence factor pthA of Xanthomonas citri subsp. citri.Transgenic research · 2022Article
- Bacterial effectors mimicking ubiquitin-proteasome pathway tweak plant immunity.Microbiological research · 2021Review
- Transcription factor WRKY22 regulates canker susceptibility in sweet orange (Citrus sinensis Osbeck) by enhancing cell enlargement and CsLOB1 expression.Horticulture research · 2021Article
- Consequences of adaptation of TAL effectors on host susceptibility to Xanthomonas.PLoS genetics · 2021Article
- The immunity of Meiwa kumquat against Xanthomonas citri is associated with a known susceptibility gene induced by a transcription activator-like effector.PLoS pathogens · 2020Article
- The MAF1 Phosphoregulatory Region Controls MAF1 Interaction with the RNA Polymerase III C34 Subunit and Transcriptional Repression in Plants.The Plant cell · 2020Article
- PthA4Molecular plant pathology · 2019Article
- A Novel Effector Protein of Apple Proliferation Phytoplasma Disrupts Cell Integrity ofInternational journal of molecular sciences · 2019Article
- HrpE, the major component of the Xanthomonas type three protein secretion pilus, elicits plant immunity responses.Scientific reports · 2018Article
- The immunophilin repertoire of Plasmodiophora brassicae and functional analysis of PbCYP3 cyclophilin.Molecular genetics and genomics : MGG · 2018Article
- Dissection of TALE-dependent gene activation reveals that they induce transcription cooperatively and in both orientations.PloS one · 2017Article
- Behind the lines-actions of bacterial type III effector proteins in plant cells.FEMS microbiology reviews · 2016Review
- Additive roles of PthAs in bacterial growth and pathogenicity associated with nucleotide polymorphisms in effector-binding elements of citrus canker susceptibility genes.Molecular plant pathology · 2016Article
- Nuclear processes associated with plant immunity and pathogen susceptibility.Briefings in functional genomics · 2015Review
- Article
- Identification of putative TAL effector targets of the citrus canker pathogens shows functional convergence underlying disease development and defense response.BMC genomics · 2014Article
- Interactions of Xanthomonas type-III effector proteins with the plant ubiquitin and ubiquitin-like pathways.Frontiers in plant science · 2014Review
- E3 ubiquitin-ligases and their target proteins during the regulation of plant innate immunity.Frontiers in plant science · 2014Review
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xanthomonas axonopodis pv. citri utilizes the type III effector protein PthA to modulate host transcription to promote citrus canker. PthA proteins belong to the AvrBs3/PthA family and carry a domain comprising tandem repeats of 34 amino acids that mediates protein-protein and protein-DNA interactions. We show here that variants of PthAs from a single bacterial strain localize to the nucleus of plant cells and form homo- and heterodimers through the association of their repeat regions. We hypothesize that the PthA variants might also interact with distinct host targets. Here, in addition to the interaction with alpha-importin, known to mediate the nuclear import of AvrBs3, we describe new interactions of PthAs with citrus proteins involved in protein folding and K63-linked ubiquitination. PthAs 2 and 3 preferentially interact with a citrus cyclophilin (Cyp) and with TDX, a tetratricopeptide domain-containing thioredoxin. In addition, PthAs 2 and 3, but not 1 and 4, interact with the ubiquitin-conjugating enzyme complex formed by Ubc13 and ubiquitin-conjugating enzyme variant (Uev), required for K63-linked ubiquitination and DNA repair. We show that Cyp, TDX and Uev interact with each other, and that Cyp and Uev localize to the nucleus of plant cells. Furthermore, the citrus Ubc13 and Uev proteins complement the DNA repair phenotype of the yeast Deltaubc13 and Deltamms2/uev1a mutants, strongly indicating that they are also involved in K63-linked ubiquitination and DNA repair. Notably, PthA 2 affects the growth of yeast cells in the presence of a DNA damage agent, suggesting that it inhibits K63-linked ubiquitination required for DNA repair.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.