ArticleTissue engineering. Part A2011
Guiding epithelial cell phenotypes with engineered integrin-specific recombinant fibronectin fragments.
Article in Tissue engineering. Part A, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- A TGFβR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cells.Scientific reports · 2022Article
- The interplay of fibroblasts, the extracellular matrix, and inflammation in scar formation.The Journal of biological chemistry · 2022Review
- The Combined Influence of Viscoelastic and Adhesive Cues on Fibroblast Spreading and Focal Adhesion Organization.Cellular and molecular bioengineering · 2021Article
- Hydrogel-Supported, Engineered Model of Vocal Fold Epithelium.ACS biomaterials science & engineering · 2021Article
- Engineering PEG-based hydrogels to foster efficient endothelial network formation in free-swelling and confined microenvironments.Biomaterials · 2020Article
- Feeling Things Out: Bidirectional Signaling of the Cell-ECM Interface, Implications in the Mechanobiology of Cell Spreading, Migration, Proliferation, and Differentiation.Advanced healthcare materials · 2020Review
- Microporous annealed particle hydrogel stiffness, void space size, and adhesion properties impact cell proliferation, cell spreading, and gene transfer.Acta biomaterialia · 2019Article
- Material Strategies for Modulating Epithelial to Mesenchymal Transitions.ACS biomaterials science & engineering · 2018Article
- Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability.Nature materials · 2017Article
- Detection of an Integrin-Binding Mechanoswitch within Fibronectin during Tissue Formation and Fibrosis.ACS nano · 2017Article
- Preventing tissue fibrosis by local biomaterials interfacing of specific cryptic extracellular matrix information.Nature communications · 2017Article
- Local remodeling of synthetic extracellular matrix microenvironments by co-cultured endometrial epithelial and stromal cells enables long-term dynamic physiological function.Integrative biology : quantitative biosciences from nano to macro · 2017Article
- Integrin α3β1 Binding to Fibronectin Is Dependent on the Ninth Type III Repeat.The Journal of biological chemistry · 2015Article
- Covalent Modification of Synthetic Hydrogels with Bioactive Proteins via Sortase-Mediated Ligation.Biomacromolecules · 2015Article
- Utilizing Fibronectin Integrin-Binding Specificity to Control Cellular Responses.Advances in wound care · 2015Review
- New Methods in Tissue Engineering: Improved Models for Viral Infection.Annual review of virology · 2014Article
- Biologically-active laminin-111 fragment that modulates the epithelial-to-mesenchymal transition in embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A missense mutation in ITGB6 causes pitted hypomineralized amelogenesis imperfecta.Human molecular genetics · 2014Article
- Activated alveolar epithelial cells initiate fibrosis through autocrine and paracrine secretion of connective tissue growth factor.American journal of physiology. Lung cellular and molecular physiology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The extracellular matrix (ECM) provides important cues for directing cell phenotype. Cells interact with underlying ECM through cell-surface receptors known as integrins, which bind to specific sequences on their ligands. During tissue development, repair, and regeneration of epithelial tissues, cells must interact with an interstitial fibronectin (Fn)-rich matrix, which has been shown to direct a more migratory/repair phenotype, presumably through interaction with Fn's cell binding domain comprised of both synergy Pro-His-Ser-Arg-Asn (PHSRN) and Arg-Gly-Asp (RGD) sequences. We hypothesized that the Fn synergy site is critical to the regulation of epithelial cell phenotype by directing integrin specificity. Epithelial cells were cultured on Fn fragments displaying stabilized synergy and RGD (FnIII9'10), or RGD alone (FnIII10) and cell phenotype analyzed by cytoskeleton changes, epithelial cell-cell contacts, changes in gene expression of epithelial and mesenchymal markers, and wound healing assay. Data indicate that epithelial cells engage RGD only with αv integrins and display a significant shift toward a mesenchymal phenotype due, in part, to enhanced transforming growth factor-β activation and/or signaling compared with cells on the synergy containing FnIII9'10. These studies demonstrate the importance of synergy in regulating epithelial cell phenotype relevant to tissue engineering as well as the utility of engineered integrin-specific ECM fragments in guiding cell phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.