ArticleMethods in molecular biology (Clifton, N.J.)2010
Flow cytometry analysis of transcription factors in T lymphocytes.
Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Enrichment of Neural Crest Cells by Antibody Labeling and Flow Cytometry for Single-Cell Transcriptomics in a Lizard.Evolution & development · 2026Article
- Detection of engineered T cells in FFPE tissue by multiplex in situ hybridization and immunohistochemistry.Journal of immunological methods · 2021Article
- LMP1+SLAMF1high cells are associated with drug resistance in Epstein-Barr virus-positive Farage cells.Oncotarget · 2017Article
- Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain.Journal of visualized experiments : JoVE · 2016Article
- Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients.Journal of inflammation (London, England) · 2015Article
- The multifaceted roles of Bcl11b in thymic and peripheral T cells: impact on immune diseases.Journal of immunology (Baltimore, Md. : 1950) · 2014Review
- TNF-α-dependent hematopoiesis following Bcl11b deletion in T cells restricts metastatic melanoma.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Article
- Transcription factor Bcl11b controls selection of invariant natural killer T-cells by regulating glycolipid presentation in double-positive thymocytes.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Detection of transcription factors in immune cell populations, particularly in subpopulations that are represented at low frequencies in lymphoid and nonlymphoid organs, presents a particular challenge when using traditional methods such as western blot analysis. Therefore, development of flow cytometry-based methods which allow identification of transcription factors in specific immune cell populations is of main interest. Here we developed and optimized a methodology for rapid and convenient detection of the transcription factor BCL11B in T lymphocyte subpopulations using flow cytometry. The optimal protocol employs saponin and Tween 20 both during the fixation and permeabilization steps, and we demonstrate that it is efficient for three anti-BCL11B antibodies covering distinctive BCL11B epitopes. In addition, we prove that the method preserves the staining of surface markers.
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