ArticleMethods in molecular biology (Clifton, N.J.)2010
Measuring the absolute abundance of the Smad transcription factors using quantitative immunoblotting.
Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Trypanosome RNAEditing Substrate Binding Complex integrity and function depends on the upstream action of RESC10.Nucleic acids research · 2021Article
- Programmable Extracellular Vesicles for Macromolecule Delivery and Genome Modifications.Developmental cell · 2020Article
- Correlation between growth differentiation factor-15 and collagen metabolism indicators in patients with myocardial infarction and heart failure.Journal of geriatric cardiology : JGC · 2016Article
- Dynamics of TGF-β/Smad signaling.FEBS letters · 2012Review
Corrections and comments
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Authors and funding
2 authors.
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Abstract
In the age of systems biology, biologists seek to quantify the absolute number of molecules in experimentally treated samples. Immunoblotting remains a technique of choice for assessing the relative differences between the protein levels in different samples. Here we discuss how to exploit immunoblotting for estimating the number of Smad transcription factor molecules per cell. We focus on describing the calculations needed to analyze the data. Our methods are generally applicable to the quantification of other cellular proteins.
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Registered trials
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