Evidence map›Paper›PMID 20694048›Full record

ArticleDigest of technical papers. International Conference on Solid-State Sensors, Actuators, and Microsystems2009

FORMING SELF-ASSEMBLED CELL ARRAYS AND MEASURING THE OXYGEN CONSUMPTION RATE OF A SINGLE LIVE CELL.

James R Etzkorn, Sarah C McQuaide, Judy B Anderson, Deirdre R Meldrum, Babak A Parviz

Open access · greenAbstract read
In one paragraph

Article in Digest of technical papers. International Conference on Solid-State Sensors, Actuators, and Microsystems, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
2.8field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

James R EtzkornElectrical Engineering, University of Washington, Seattle, WA, USA.
Sarah C McQuaide
Judy B Anderson
Deirdre R Meldrum
Babak A Parviz
University of Washington · USArizona State University · US

Funding

CEGSTech: Integrated Biologically-Active MicrosystemsP50HG002360 · NHGRI · UNIVERSITY OF WASHINGTON · PI MELDRUM, DEIRDRE R. · 2001 to 2012
$37.8M
NHGRI NIH HHS P50 HG002360
6 · The paper itself

Abstract

We report a method for forming arrays of live single cells on a chip using polymer micro-traps made of SU8. We have studied the toxicity of the microfabricated structures and the associated environment for two cell lines. We also report a method for measuring the oxygen consumption rate of a single cell using optical interrogation of molecular oxygen sensors placed in micromachined micro-wells by temporarily sealing the cells in the micro-traps. The new techniques presented here add to the collection of tools available for performing "single-cell" biology. A single-cell self-assembly yield of 61% was achieved with oxygen draw down rates of 0.83, 0.82, and 0.71 fmol/minute on three isolated live A549 cells.

Identifiers

PMID20694048
PMCPMC2916695
OpenAlexW2152989967

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.